MUSCULAR TISSUE. 



77 



The striated fibres of the heart differ from the voluntary stri- 

 ated fibres in being devoid of a sarcolemma and in having their nu- 

 clei centrally located. 



Laboratory exercise No. 23. Striated muscle. Harden pieces of vol- 

 untary muscle of salamander or cat in alcohol, embed in paraffin, and 

 stain with carmine, method No. 3. Make two sections, longitudinal and 

 transverse. 



L.S. Observe the fibres running parallel with each other. Using the 

 high power, demonstrate the stria tions of the fibres dark, lateral, and 

 intermediate. Examine the ends of the torn fibres and search for the 

 ultimate fibrils. Try and demonstrate the sarcolemma. The tissue of 

 a salamander will give the best results. 



T.S. Examine a section of an entire muscle, if possible, and demon- 

 strate the epimysium, perimysium, and endomysium. Focus upon the 

 end of a fibre and locate the nucleus near the outer surface. What are 

 Cohnheim's areas? 



Incubator. 



Bausch & Lomb Optical Co., Rochester, N. Y. 



