156 BACTERIOLOGY. 



CHAPTER XXVI. 



MICROSCOPIC TECHNIQUE. 



I. REAGENTS AND STAINS. 



(1) DECOLORIZING SOLUTIONS. 



Twenty-five per cent aqueous solutions of hydrochloric, nitric, 

 and sulphuric acids may be used for decolorizing. 



(2) ACID ALCOHOL. 



Hydrochloric acid 1 part 



Alcohol (seventy per cent) 100 parts 



(3) IODINE SOLUTION. 



Iodine 1 gram 



Potassium iodide 2 grams 



Water 300 cc. 



(4) CARBOL FUCHSIN. 



Fuchsin 1 cc. 



Alcohol 10 cc. 



Dissolve and add 10 c. c. of five per cent solution of carbolic acid. 

 Filter. 



(5) ACID METHYLENE BLUE. 



Sulphuric acid 16 cc. 



Water 90 cc. 



Methylene blue 2 grams 



This stain should be prepared fresh from time to time. The 

 carbol fuchsin improves with age. 



(6) LOFFLER'S ALKALINE METHYLENE BLUE. 



Concentrated alcoholic solution of methy- 



lene blue 30 c. 



Potassum hydrate (Aq. Sol., 1-10,000) 100 cc. 



This is especially useful in staining the baccillus of diphtheria. 



(7) ANILINE-WATER GENTIAN-VIOLET. 



Aniline oil 5 cc. 



Water.. ..100 cc. 



