

MICROSCOPIC TECHNIQUE. 159 



Staining Tissues for Bacteria. 



Tissues may be stained by Gram's method or by the following 

 process : 



Method No, XVI. Method for Staining Bacteria in Sections, 



(a) Using an aqueous solution of fuchsin, gentian-violet or methy- 

 lene blue, apply stain for about five minutes. 



(b) Wash in water. 



(c) Apply an aqueous solution of acetic acid, one per cent, for one 

 minute. 



(d) Apply alcohol one to two minutes. 



(e) Clear up with xylol. 



(f ) Mount with balsam. 



MEMORANDA. 



