168 BACTERIOLOGY. 



Laboratory exercise No. 60. Micrococcus gonorrhaxv. Make a cover- 

 glass preparation from gonorrhceal discharges and stain with Loffler's 

 methylene bine or carbol fuchsin, method No. 10. The hemispherical 

 cocci will be found in pairs or tetrads on epithelial cells or within pus 

 cells. 



Laboratory exercise No. 61. Bacillus tuberculosis. Make a rather 

 thick smear of tubercular sputum upon a cover-glass, dry thoroughly, 

 and stain by method No. 15. Observe the slender, beaded, somewhat 

 curved rods. Find two attached by their extremities forming a V-shape. 

 Account for the beaded appearance. 



Laboratory exercise No. 62. Bacillus typhosus. Make a systematic 

 study of this species and prepare an outline of your work. Observe the 

 motility of vegetative specimens. Demonstrate flagella, method No. 13. 

 Make a permanent preparation, staining with gentian-violet. Look for 

 small oval spaces in the ends of some of the degenerated bacilli. 



Laboratory exercise No. 63. Bacillus anthracis. Make, a systematic 

 study of this species and prepare an outline. Stain a permanent prepa- 

 ration with gentian-violet. Make a study of the long filaments. Dem- 

 onstrate spores by method No. 12. Harden, embed, and section the 

 heart and lungs of a mouse that has been killed by Bacillus anthracis, 

 and stain by method No. 16. Search for bacteria. 



Laboratory exercise No. 64. Bacillus coli commune. Make a sys- 

 tematic study of the colon bacillus. State all the points of difference 

 between this species and Bacillus typhosus. Stain your permanent 

 preparation with fuchsin or gentian-violet. Make drawings of all spe- 

 cies studied. 



Laboratory exercise No. 65. Bacillus diphtheriae. Make a study of 

 cultures of the diphtheria bacillus on different media. Describe the 

 process of making a bacteriological diagnosis of diphtheria. Stain a 

 cover-glass preparation with Loffler's methylene blue and make a study 

 of the cells. Observe the dumb-bell forms. A good lens will always 

 exhibit complete rods, showing that the protoplasm of the polar ends 

 is connected. Search for involution forms, also for three or four cells 

 joined by their extremities, noting that no chains are formed. Draw- 

 ing. 



