TABLE OF CONTENTS. 



PART I. 

 CHAPTER I. 



BY THOMAS E. SATTERTHWAITE, M.D. 



MATERIALS REQUISITE FOR HISTOLOGICAL WORK. How TO USB THE MICRO- 

 SCOPE. TESTING THE MICROSCOPE. ITS USES Page 1 



Appliances for microscopic work, 1. Chemical reagents, 3. Illumina- 

 tion, 4. Stage diaphragms, 5. The mirrors, 5. Direct and oblique light, 5. 

 Arrangement of the object, 6. Kind of lens to use, 6. How to keep the 

 instrument clean, 7. Magnifying power of a lens, 7. How to estimate the 

 size of an object, 8. Testing a lens, 8. How to illuminate the object, 9. 

 Testing the eye-piece, 10. Testing high lenses, 10. Measuring the angle of 

 a lens, 10. 



CHAPTER II. 



BY THOMAS E. SATTERTHWAITE, M.D. 



METHODS FOR PREPARING MICROSCOPIC OBJECTS Page 12 



General directions, 12. To prepare fresh objects for rapid examination, 

 13. Ordinary methods of preparing tissues, 13. Mailer's fluid, 14. Potas- 

 sium bichromate solution, 14. Ammonia bichromate solution, 14. Alcohol 

 and acetic acid mixtures, 14, 15. Molybdate of ammonia, 15. Solution of 

 osmic and chromic acids, 15. Alcohol and acetic acid, and muriatic acid 

 solution, 15. Method of hardening the brain, 15. How to embed speci- 

 mens, 15. Embedding in glycerine and tragacanth, 16. The hand section- 

 cutter, 16. Freezing section-cutters, 17. Hailes' microtome, 19. The 

 Vincent microtome, 21. Staining fluids, ammonia carmine, 21. Borax car- 

 mine, 22. Double staining, 22. HEematoxylon solutions, 23, 24. Solutions 

 for multiple staining, 24, 25. Preparation of the cornea, 25. Triple stain- 



