ALCOHOLIC FERMENTATION OF SUGAR 



141 



The fermenting mixture is contained in the round- 

 bottomed flask, placed in a constant temperature water- 

 bath. The C0 2 is collected over mercury in the graduated 

 burette, a constant pressure being maintained by the simple 

 compensating arrangement shown in the diagram. 



Table III will illustrate the results already described : 



TABLE in 

 (i) The effect of the addition of boiled juice 



CO 2 evolved in presence of 



(ii) Experiment on the filtration of yeast juice : 



C0 2 evolved in presence of 



The question now remains, what is the nature of the 

 dialysable matter, or co-ferment, as it has been termed, 

 which is necessary for the activity of the enzyme, which 



