IDENTIFICATION OF BACTERIA 63 



(6) Inoculations are occasionally made into the anterior cham- 

 ber of the eye, the pleura, and the cranium. In certain 

 experiments animals are made to inhale dust or infected 

 spray, in others the infective material may be given in 

 food or passed into the intestines by means of a rubber 

 tube. 



Autopsy on Dead Animal. The autopsy should be made as 

 soon as possible after death. If an interval occurs the animal should 

 be kept at a temperature between 1 C. and 4 C. For small 

 animals, the procedure is as follows : The body is wiped with a 1 

 in 20 carbolic acid solution and stretched out back downwards 

 on a shallow metal trough having a perforation at each end through 

 which a tape or cord may be passed. Two sets of sterile forceps, 

 scissors, and scalpels and several sterile Petri dishes should be ready. 

 The skin in the median line of the pelvic region is lifted with for- 

 ceps, and with the scissors an incision is made through the skin, 

 only upwards to the neck. From the ends of this median incision 

 the skin is cut outward toward each leg and drawn back with 

 forceps. If the injection has been subcutaneous the neighboring 

 lymph nodes are examined, and if abnormal they are removed 

 with sterile scissors and forceps and placed in a Petri dish to await 

 further examination. An incision is then made through the ab- 

 dominal wall, the diaphragm is freed, and the thorax is opened by 

 cutting through the ribs on both sides of the sternum. Films 

 and cultures are made from any exudates and from diseased organs. 

 To obtain heart blood the tip of the left ventricle is seared with a 

 red-hot spatula, an incision is made through the seared area, and 

 the blood removed with a capillary pipette or a platinum loop. 



When the examination is finished the body should be immedi- 

 ately burned. The instruments should be boiled in a 3 per cent 

 sodium carbonate solution for half an hour, and the dissecting 

 trough may be allowed to stand twenty-four hours in a 1 in 20 

 carbolic acid solution. 



