24 DAVID J. DAVIS 



cal irritation. Czaplewski states that, while working with his 

 organism, he was taken with a coryza accompanied with some cough, 

 which lasted for about a week. He obtained his bacillus from his 

 nose and throat in large numbers. 



Human inoculation. As no attempts are on record to inoculate 

 man directly with the influenza-like bacillus from pertussis, it seemed 

 desirable to make such a test with the view of determining its pos- 

 sible pathogenicity. A young man, who, according to his statement 

 and also that of his relatives, had never had whooping-cough, agreed 

 to submit to inoculation with hemophilous organisms isolated from 

 the pertussis cases. This was done with his full consent, and he was 

 informed concerning the character of the material inoculated and the 

 possible results anticipated. He was healthy in every way, and an 

 examination of his throat made previous to the inoculation showed 

 only the usual bacterial flora of the normal throat. There were no 

 influenza-like organisms present. Pure blood-agar cultures, 24 hours 

 old, of influenza-like bacilli, isolated from an uncomplicated case of 

 whooping-cough, were smeared upon the nasal mucosa and the ton- 

 sils. On the second day, almost exactly 48 hours after the inocula- 

 tion, the patient complained of a chilly sensation, a cold sweat, some 

 headache and weakness. His temperature rose from normal 

 (98. 4 F. the day previous) to ioo.2F. The same evening it 

 registered ioo.iF. The next morning it was normal again, but 

 rose during the day to 99.5. The third day his temperature was 

 practically normal and continued so from that time on. He com- 

 plained somewhat of headache and of feeling unwell, and on the 

 second morning said he coughed a little. His throat, upon examina- 

 tion when the first symptoms appeared, was slightly hyperemic; the 

 next morning there was present a thick layer of mucus upon the 

 pharyngeal wall, and from this time on he coughed, or rather hacked 

 up large quantities of a stringy, tenacious mucus. This condition 

 continued, becoming gradually less marked, and was still apparent 

 at the end of four weeks. After the first few days he felt perfectly 

 well, complaining only of slight discomfort in the throat and the light 

 cough. This cough was not spasmodic and did not resemble 

 whooping-cough. The leucocyte count on the second day was 9,200. 



Bacteriological examination of the mucus from the throat, obtained 



