108 LABORATORY BACTERIOLOGY 



EXERCISE XLVII 



ISOLATING AND IDENTIFYING BACTERIA FROM 

 ANIMAL TISSUES 



158. In making a bacteriologic investigation into the cause 

 of death in an animal or man it is necessary to make cultures 

 from the various organs and the blood to find whether or 

 not there are any pathogenic or other bacteria present. This 

 necessitates a knowledge of making cultures from animal tis- 

 sues. In this exercise an experimental animal (rabbit or 

 guinea pig) which has died from some bacterial disease will 

 be provided. The purpose of this examination is to find out 

 what that disease is. To save animals, each student will make 

 cultures from but one organ. From time to time during the 

 course opportunity will be afforded for making cultures from 

 variously diseased animal tissues. 1 Each student will be given 

 opportunity to inoculate one or more animals some time during 

 the course. 



159. Work for this exercise. The experimental animal fur- 

 nished will be tied out on a post-mortem tray and the viscera 

 exposed. (Directions for the post-mortem examination will 

 be given in the course in pathology.) 



Inoculate a tube of bouillon, one of agar, and a fermenta- 

 tion tube of glucose bouillon from either the liver, spleen, or 

 kidney. (In an actual investigation of an unknown disease, 

 cultures should be made from all of the organs, blood, and 

 lymphatic glands.) 



Make a series of 3 agar plate cultures from the same organ. 



Make and examine 2 cover-glass preparations from the 

 organ from which the cultures were made. Stain one with 

 alkaline methylene blue and one with carbol fuchsin. (It is 



1 For methods of inoculating animals for purposes of diagnosis, see 

 Appendix III. 



