BACTERIOLOGICAL DIAGNOSIS I2/ 



EXERCISE LVIII 



BACTERIOLOGICAL DIAGNOSIS 



183. This and the following exercises of this course will be 

 devoted to the application of the methods already studied to 

 practical diagnostic work, or to the making of some examina- 

 tion or examinations, or to carrying out some little investiga- 

 tion that seems best suited to the needs of the student. Just 

 what these will be must be decided by the instructor at the 

 time. We have found very helpful the diagnosis of tuberculosis, 

 diphtheria, anthrax, pyogenic bacteria, and others from mixed 

 cultures or animal tissues. If the student is to continue work 

 in bacteriology, a study of the varieties of some group of bac- 

 teria or of some bacterial flora may be better. It is important 

 that the student should learn something of the possibilities and 

 limitations of the methods in practical bacteriological work. 



184. Work for this and the following exercises. From the 

 material furnished make such examinations, cultures, or other 

 tests as in your opinion are called for to make the determina- 

 tions required. 



Make careful notes on the work and state fully in your 

 report the reasons for the conclusions reached. 



At or before the last exercise have all apparatus for indi- 

 vidual use inspected by the instructor and returned to the 

 laboratory. 



185. Staining actinomyces. This fungus is stained in cover- 

 glass preparations made from actinomycotic tissue, or in sec- 

 tions by any of the basic aniline dyes. Carbol fuchsin is very 

 good. The Gram method gives very excellent results. It is, 

 however, not easy to obtain nicely stained preparations show- 

 ing both the " clubs " and the mycelial part of the fungus. 



186. Staining blood films for malarial parasites. Several 

 methods of staining blood films to demonstrate malarial 



