

Mil M 





mid-intermediate in the reaction with iodine. In the 



polariz*tiuu niul t. IHJK rature reactions it is clo*.T tu t.'i. 



. pan nt, mill tn tli<- gentian-Mnlei and ufnuin 



. r In the seed parent. 



Table A t!u- rcactnm-intr: p<Tcen- 



tages of total -t.ii.h gelatinized at definite intends 

 BOMS). 



\ I I " irY-UUCTION COIVKS. 



Tins se. tin ti. lit- of tin' velocity- reaction curve* of 

 the starches of lltppfastnim ossullan. 11. pyrrha, and 

 iulian-iiyrrlui. .-howm-; the quaiitiUtn. differences 

 in th<> l-ehauor toward different reagents tt definite time- 

 r\aJs. (I 'harts 1) i:t to D03.) 

 Tl features of these chart* do not differ 



in man. r. -;>. , :- from those of the preceding net 



( 1 i 'lii.- . urves of all three starches are in all of the 

 close ami, <>n the whole, about the tame as 

 regard* the extent of separation as in the fint set, in 

 tlii-ri- !..-in^ a little more separation and 

 in other* lee*. In most of the reactions then is a ten- 

 dency fr a slightly higher reactivity than in the //. 

 M'.'.iri-r/cvfita set Many of the reactions are so slow 

 that there is no important if any differentiation, as in 

 with potassium sulphide, sodium sulphide, cahium 

 nitrate, uranium nitrate, strontium nitrate, cobalt ni- 

 ;<r nitrate, cupric chloride, barium chloride, 

 and mercuric chloride. 



. Omitting these very slow reactions, the curve 

 <>f //. ossultan is in the remaining 11 reactions higher 

 than the corresponding curve of the other parent in 

 the reactions with chloral hydrate, chromic acid, nitric 

 ai ul, potassium iodide, potassium sulphocyanate, sodium 

 ii\'h'.\:.!e, and sodium salicylate ; and lower in those with 

 pyrogallic acid, sulphuric acid, hydrochloric acid, and 

 potassium hydroxide. 



> The curves of the hybrid bear varying relations 

 parental carves, with very little tendency to same- 

 ness in relation to the teed parent and none to the 

 pollen parent; with little tendency to in termed iateness 

 r t<> In'iiig the lowest of the three curves; with a marked 

 to be the highest of the three ; and with a ten- 

 clciii v to sameness an both parents in the reactions that 

 take place with marked slowness. (See the following 

 section.) 



( 4 ) An early period of comparatively high resistance 



especially in the reactions with chloral hydrate, 

 ;iic acid, nitric acid, hydrochloric acid, and potas- 

 sium sulphocyanate; the opposite with potassium hy- 

 droxide and sodium salicylate. 



(5) The best period for the differentiation of the 

 three starches is in case of the very slow reactions above 



red to at the end of the 60 minutes, but in some of 

 them even at this time there is very little or no differ- 

 ence. The curves appear to be best separated at 5 min- 

 utes in the reactions with sulphuric acid, potassium hy- 

 droxide, and sodium salicylate; at 15 minutes with 

 chloral hydrate, chromic acid, pyrogallic acid, and so- 

 dium hydroxide; at 30 minutes with nitric acid, hydro- 

 chloric acid, and potassium sulphocyanate. 



REACTION-INTENSITIES OF THE HYBRID. 



This section treats of the reaction-intensities of the 

 hybrid as regards sameness, intennediatenen, execs*, and 

 t in relation to the parents. (Table A 3 and Charts 

 1)43 toD63.) 



The reactivities of the hybrid are the same as those 

 of the seed parent with sulphuric acid, sodium sulphide, 

 and uranium nitrate; the same as those of the pollen 

 parent in none ; the same as those of both parent* with 

 potassium sulphide, calcium nitrate, strontium nitrate, 

 cobalt nitrate, copper nitrate, mprio chloride, barium 



i hlonde, and men un< mediate with 



iiHline, chloral hydrate, and Midium h\di u Uw 



first being mid intermediate and in the last two nearer 

 the aead parent) ; highest with polarization, gentian vio- 

 let, safranin, temperature, chromic acul acid, 

 pyrogallic and, hydro* lilon, a. id, potasniuni hy.lr. 

 potaasiuiu iodide, and potassium nulphocyamtt. 

 being closer to the seed parent and in five being closer 

 to the pollen parent) ; and the lowest with sodium sal icy- 

 lute, it U mg in the** nearer the pollen pa 



The following is a summary of the reaction 

 ties: Same a* seed parent, 3; same a* pollen paren 

 same as both parent*, 9 ; intermediate, 3 ; highest, 1 1 , 



lowest, 1. 



In not a single reaction is there sameness in relation 

 to the pollen parent, and the stronger inlluenee of tin- 

 Mad parent on the properties of the hybrid is quite 

 marked. Intenneiliateness is rather exceptional, a 



to the lowest reactivity very exceptional, and a 

 tendency to the highest reactivity very marked. 



COMPOSITE CURVED or TUB KKACTI<>S 



This section treat* of composite curves of the reac- 

 tion-intensities showing the differentiation of the 

 starches of llippeastrum ouultan, II. pyrrha, and //. 

 oftultan-pyrrlia. ( ( 'hart E 3. ) 



Among the conspicuous features of this chart are : 



(1) Tne remarkable closeness of all three curves, 

 the differences for the most part !-m_' in-i^intii ant r 

 actually falling within the limit- of error of e\|.. rm,.-:it. 

 showing an extreme botanical closeness of the parents 

 and extremely little variance of UK- hyhrid from the 

 parents. The only reactions in which the parents are 

 readily differentiated are those with iodine, gentian 

 violet, safranin, temperature, chromic acid, and sodium 

 salicylate, and even in these the difference* are without 

 exception of a minor degree. 



(2) In this curve of //. ostultan compared with that 

 of //. pyrrha the reactivities are shown to be di-tnn tly 

 higher in the reactions with gentian uolet, safrajim, 

 chromic acid, and sodium salicylate, and lower with 

 polarization, iodine, and temperature. In the other in- 

 stances the differences are unimportant or even negligible 

 excepting in so far as they tend to indicate a generally 

 slightly higher reactivity of //. ouultan. 



(3) In //. oMullan the very high reactions with 

 polarization, chromic acid, sulphuric acid, and mdinm 

 salicylate, the moderate reactions with iodine, safranin, 

 gentian violet, and pyrogallic acid ; the low rea 

 with temperature, nitric acid, hydrochloric acid, potas- 

 sium hydroxide, and (.otasKium sulphocyanaU' ; and the 

 very low reactions with chloral hydrate, potassium iodide, 

 potassium sulphite, sodium hydroxide, sodium sulphide, 

 calcium nitrate, uranium nitrate, strontium nitrate, co- 

 balt nitrate, copper nitrate, cupric chloride, barium 

 chloride, and mercuric chloride. 



(4) In //. pyrrha the very high reactions with polari- 

 zation, sulphuric acid, and sodium salicylate; the high 

 reactions with chromic acid, the moderate reactions with 

 iodine, gentian violet, safranin and pyrogallic aenl 



low reactions with temperature, nitric arid, hydrochloric 

 acid, potassium hydroxide, potassium sulphocyanate; and 

 the very low reactions with chloral hydrate, potassium 

 iodide, potassium sulphide, sodium hydroxide, sodium 

 sulphide, calcium nitrate, uranium nitrate, strontium 

 nitrate, cobalt nitrate, copper nitrate, cupric chloride, 

 barium chloride, and mercuric chloride. 



(5) In the hyhrid the very high reaction* with polar- 

 ization, chmmn arid, sulphuric acid, pyrogallic acid, and 

 -..hum xalitvlate; the moderate reaction* with iodine, 

 gentian violet, safranin, temperature, and hydrochloric 



