. \i \\ mi 



137 



with polariration anil .-.ifranm ; and the 



moderate reactions with i.lme. gentian \iol.-t, t. m- 



.r- . ), r ._ ,!h, a, i,l. nitric acid, and potassium 



1 .. a summary of the reaction-intensities: 



^0!8 09 THE STARCHES OF CALANTHK 

 TMTH \ v.\K. Rl'BRO-OCfLATA, C. REOMKRI, AND 



I 



In tli.- hit.>logic characteristic*, polariscopic figures, 



-.vith sclenile, qualitative reaction* with iodine, 



itn.l .(iialitatne reactions with the various chemical rea- 



.!.. starches of parenU and hybrid exhibit proper- 



ii common in varying degree* of development and 



in ea. h ease more or lea marked individualities. The 



hybrid C. liryan is in form in the majority of the grains 



jnirri. and in a minority of the grains 



('. f-stita var. rubro-ocvlata. In hilum and 



lamella? it is closer to C. regnitri. In moan size the 



grains are larger than those of either parent but closer 



rrijnu-ri. while in proportion of length to width 



m- closer to the other parent. In polariscopic figure 



ami reaction* with selenite it is closer to C. regnifri. 



In th<- i|iialitati\i> reactions with iodine it is closer to 



/Mi.rt. In the qualitative changes daring heat 



L'<-latiiii/.iii"ii it is, during the first stages, closer to ' '. 



rvfmrri, but (hiring the later stages closer to the other 



parent. In the qualitative reactions with chloral hydrate, 



i lir.'imr .!;!. nitric acid, and sodium salicylate it is closer 



ir. rubro-orvlata. but in those with hydro- 



chluric acid and sodium salicylate it is closer to C. 



rignirri. 



Kr*ftto*-imtrn*ittrs Erfrrueii by Light, Color, and Tempera- 

 ture Reaction*. 

 PolariiatMw: 



C. veat. T. nibco-oe.. moderato to very high, value 70. 

 C. recnieri, very low to very hich. much lower than in C. veetita 



var. ruliro-orulaU. value 36. 

 ryan, very low to very hich. intermediate between the parent*. 



value 45. 

 e: 



.ret. v. nibro-oc.. moderate, value 50. 

 enieri. moderately light, lichter than in C. ve*tiU var. rubro- 



oewate, value 35. 



ryan. moderate, intermediate between the parent*, value 38. 

 Gentian violet:' 



-t. v. rubro-oe., moderate to deep, value 00. 



cnieri. licht to moderately deep, lichter than in C. vortiU var. 



rubro-oculaU, value 50. 

 ryan. moderate to moderately deep, intermediate between 



parrot*, value 63. 

 Safranin: 



C. veet. v. rubro-oe.. moderate to moderately deep, value 85. 

 C. rrcnirri. moderate to moderately deep, lighter than in C. veetiU 



var. nibro-oculata. value 00. 

 C. bryan. moderate to moderately deep, intermediate between the 



parent*, value 83. 

 Temperature: 



C. vcet. v. rubro-oc.. in the majority at 72 to 74*. in all at 74 to 75*. 

 mean 74.5*. 



cnit n. in the majority at 70 to 72*. in all but rare (rains al 



70 to 78* , mean 77*. 



ryan. in the majority at 72 to 74. in all l.ut rare (Tain* at 70 tu 

 77. mean 70.6-. 



C. vtsltla var. rubro-oculata exhibit* a higher reartiv- 

 itv than the other parent in all five reactions, the diller- 

 fiice being very marked in the polarization reactions. 

 slight in those with temperature; ami little in the <>th. r- 

 The hybriil C. liri/nn has intermediate reactiutii-s be- 

 tween the parents in all of the reaction*, being generally 

 somewhat closer to (\ vtttita var. ruliro-nrulata than to 

 the other parent. 



Table A 4(> show* the reaction-intenoitir* in jx-rrent- 

 ages of total starch gelatinized at definite intervals (i 

 onds and minutes) : 



TABU A 40. 



VKLOCITT-RKACTIOW CCHTM. 



This section treats of the velocity-reaction carves of 

 the starches of Calanllie vrstita var. rubro-oculata, C. 

 regnitri, and C. bryan, showing the quantitative differ- 

 ences in the behavior toward differ, nt reagents at definite 

 time-intervals. (CharU 1) CK? to \> ti^l.) 



Among the most conspicuous feature* of these charts 

 are : The generally close correspondence in the course* of 

 all three curves. The well-marked separation of the 

 parental curves, even in thr sulphuric-acid reaction*, 

 which occur very quickly, there being a* high a gelati- 

 nization of one parent in one-half a minute as in the 

 other in 5 minutes. The curve of C. rtslila var. rubro- 

 oculala is lower than the carve of the other parent in all 

 of the 8 reactions. The curves of the livhrid show a 

 very marked tendency to intcrmediatencss, and when not 

 mid-intermediate the inclination seems to be in r.- 

 marked toward the pollen parent. In other reaction*, in 

 one there is sameness, in relation to the seed parent and 

 in another the hybrid re.-tii>n i* the highest of the thr.-e 

 and nearer the pollen parent. A tendency to an early 



