AMARYLLIS BRUNSVIGIA. 



391 



sure than in A. belladonna, but very much less frequently 

 than in Brunsvigia josepliince. More than one bubble 

 sometimes appears at the hilum, somewhat more fre- 

 quently than in A. belladonna, but less frequently 

 than in Brunsvigia, josephince. The definition of 

 the lamella, the appearance of the refractive border, 

 the method of gelatinization, and the most resistant area 

 in the grains are about the same in most of the grains 

 as in A. belladonna, yet in some grains a resemblance to 

 Brunsvigia josephince is quite marked, notably in the 

 gelatinization of the entire border of some grains before 

 progress is made in the main body of the grain, in the 

 sharper definition of the lamella? in a few grains, and in a 

 greater distortion of the capsule in a few grains. The 

 gelatinized grains are swollen and usually distorted more 

 at the proximal than the distal end, they do not resemble 

 the form of the untreated grain ; slightly more distortion 

 in some grains than in A. belladonna, but considerably 

 less than in Brunsvigia josepliince. In this reaction 

 Brunsdonna sanderce alba shows qualitatively a much 

 closer relationship to A. belladonna than to Brunsvigia 

 josephincB. 



The reaction with calcium nitrate begins in rare 

 grains in half a minute. Complete gelatinization occurs 

 in less than 0.5 per cent of the grains and over 4 per 

 cent of the total starch in 5 minutes ; and in over 4 per 

 cent of the grains and about 22 per cent of the total 

 starch in 15 minutes; in about 6 per cent of the grains 

 and 30 per cent of the total starch in 30 minutes ; in over 

 13 per cent of the grains and about 36 per cent of the 

 total starch in 45 minutes ; and in about 23 per cent of the 

 grains and 41 per cent of the total starch in 60 minutes. 

 (Chart D 14.) 



The reaction with uranium nitrate begins in rare 

 grains in half a minute. Complete gelatinization occurs 

 in about 0.5 per cent of the grains and 2 per cent of the 

 total starch in 5 minutes; in about 2 per cent of the 

 grains and 7 per cent of the total starch in 15 minutes; 

 in about 2 per cent of the grains and 15 per cent of the 

 total starch in 30 minutes; in about 14 per cent of the 

 grains and 30 per cent of the total starch in 45 minutes ; 

 and in about 21 per cent of the grains and 50 per cent 

 of the total starch in 60 minutes. (Chart D 15.) 



The reaction with strontium nitrate begins imme- 

 diately. Complete gelatinization occurs in about 23 per 

 cent of the grains and 72 per cent of the total starch 

 in 5 minutes; in about 87 per cent of the grains and 97 

 per cent of the total starch in 15 minutes ; and in about 

 97 per cent of the grains and over 99 per cent of the total 

 starch in 30 minutes. (Chart D 16.) 



The reaction with cobalt nitrate begins in rare grains 

 in 1 minute. Complete gelatinization occurs in less than 

 0.5 per cent of the entire number of grains and 2 per cent 

 of the total starch in 5 minutes; slight progress in 15 

 minutes; still less than 0.5 per cent complete of the 

 entire number of grains, and 3 per cent of the total 

 starch in 30 minutes ; about the same in 45 and 60 min- 

 utes, respectively. (Chart D 17.) A group of irregu- 

 larly arranged fissures may form in the region of the 

 hilum, and these may extend through the grain, still re- 

 taining their irregularity. This may be followed by gela- 

 tinization starting at the proximal end and proceeding 

 distalwards, the most resistant area being located near 

 and at the distal margin. In the few grains which 



showed any progress with this reagent the relationship 

 appeared closer to A. belladonna than to Brunsvigia 

 josepliince. 



The reaction with copper nitrate begins in rare grains 

 in half a minute. Complete gelatinization occurs in but 

 rare grains, less than 0.5 per cent of the entire number of 

 grains and total starch in 5 minutes; complete gela- 

 tinization occurs in about 0.5 per cent of the entire num- 

 ber of grains and 2.5 per cent of the total starch in 15 

 minutes ; in about 1 per cent of the grains and 6 per cent 

 of the total starch in 30 minutes ; in about 2 per cent of 

 the grains and 10 per cent of the total starch in 45 min- 

 utes; in about 3 per cent of the grains and 18 per cent 

 of the total starch in 60 minutes. (Chart D 18.) Gela- 

 tinization in most of the grains proceeds from the proxi- 

 mal end to the distal margin as noted in A. belladonna; 

 but in a few grains deep fissures are formed, along the 

 course of which the process proceeds as noted more com- 

 monly in Brunsvigia josephince, although even in these 

 grains the most resistant area is more frequently located 

 at the proximal end. In the few grains affected by the 

 reagent, these reactions exhibit a closer relationship to 

 A. belladonna than to Brunsvigia josephince. 



The reaction with cupric chloride begins in rare 

 grains in 1 minute. Complete gelatinization was ob- 

 served in less than 0.5 per cent of the entire number of 

 grains and the total starch in 5 minutes ; in about 0.5 per 

 cent of the grains and 2.5 per cent of the total starch in 

 15 minutes; in about 1 per cent of the grains and 6 per 

 cent of the total starch in 30 minutes ; in about the same 

 percentage of the grains and 7.5 per cent of the total 

 starch in 45 minutes ; in about 2 per cent of the grains 

 and 10 per cent of the total starch in 60 minutes. (Chart 

 D 19.) Irregular fissures appear in the area around the 

 hilum and in some grains also at the distal margin. The 

 process then generally proceeds from the proximal end 

 and gradually advances towards the distal margin. The 

 progress of the reaction more closely follows that of A. 

 belladonna than of Brunsvigia josepliina', but the appear- 

 ance of fissures is more frequent than in these species. 

 In the few grains affected by the reagent, the relationship 

 appears closer to A. belladonna than to Brunsvigia 

 josephince. 



The reaction with barium chloride begins in rare 

 grains in 1 minute. Complete gelatinization occurs in 

 less than 0.5 per cent of the grains and total starch in 

 5 minutes; and in about 0.5 per cent of the grains and 

 total starch in 15, 30, 45, and 60 minutes, respectively. 

 (Chart D 20.) 



The reaction with mercuric chloride begins in very 

 rare grains in 1 minute. Complete gelatinization occurs 

 only in rare grains and the process has begun in very few 

 grains, less than 0.5 per cent of both the entire number 

 of grains and of the total starch in 5 minutes ; very little 

 progress is observed in 15 minutes; still less than 0.5 per 

 cent of the entire number of grains and about 0.5 per cent 

 of the total starch in 30 minutes ; about the same in 45 

 and 60 minutes, respectively. (Chart D 21.) 



BEUNSDONNA SANDEEO; (HYBRID). 



HISTOLOGIC PROPERTIES. 

 (Plate 1, fig. 6; Charts D 1 to D 21.) 



The form of the grains is usually simple and isolated 

 with the exception of a few which occur in aggregates 



