HIPPEASTRUM. 



417 



of gelatinization are observed as those noted for the 

 parents. The process more frequently starts in the 

 border either at one or several points following very deep 

 striation than in the parents, but nearer to // . ossultan. 

 In addition to the methods of gelatinization noted in the 

 parents, a mass of interlacing fissures may follow the 

 punctation or pitting of the grain and the lamellae be 

 quickly disorganized into very refractive granules. The 

 lamella of all the grains are usually disorganized into 

 refractive granules previous to gelatinization as in the 

 parents. These granules are less resistant than in the 

 parents, but nearer the resistancy of H. ossultan, the most 

 resistant starch, however, being located as in the parents. 



The gelatinized grains are swollen and slightly to 

 considerably distorted, not as much distorted in so many 

 grains as in H. ossultan, but more than in H. pyrrha. 

 Refractive granules remain in a number of otherwise 

 completely gelatinized grains; about the same as in H. 

 ossultan, but less with refractive granules than in 

 H. pyrrha. Many grains are but little affected beyond 

 the swelling of the hilum, but gelatinization is further 

 advanced in a larger percentage of grains than in the 

 parents, but nearer to H. ossultan. 



In this reaction 77. ossultan-pyrrha shows qualita- 

 tively a very close relationship to both parents, but 

 slightly nearer to //. ossultan. A character inherited 

 from one parent is often developed further in the hybrid. 



The reaction with potassium sulphocyanate begins 

 in a few grains in half a minute. Complete gelatiniza- 

 tion occurs in about 2 per cent of the entire number of 

 grains and 3 per cent of the total starch in 5 minutes ; in 

 about 3 per cent of the grains and 10 per cent of the total 

 starch in 15 minutes; in about 23 per cent of the grains 

 and 48 per cent of the total starch in 30 minutes ; in about 

 35 per cent of the grains and 61 per cent of the total 

 starch in 45 minutes ; in about 50 per cent of the grains 

 and 70 per cent of the total starch in 60 minutes. 

 (Chart D 51.) 



The hilum enlarges and becomes very distinct as in 

 the parents, and the fissuring is nearly the same as in 

 H. ossultan, except that the fissures are more branched, 

 but not so branched nor so irregular as in H. pyrrha. 

 The lamellae gradually become very distinct and in some 

 grains remain so, but in others are obscured as in H. 

 ossultan. Gelatinization begins at the hilum as in 

 H. ossulian, and never at both hilum and margin as in 

 some grains of //. pyrrha. The progress of gelatiniza- 

 tion is the same as in //. ossultan, except that there are 

 not so many in which fissuration and granulation of one 

 segment of the grain occur as in //. ossultan, but more 

 than in //. pyrrha. The gelatinized grains have the same 

 appearance as those of the parents. 



In the reaction with potassium sulphocyanate H. 

 ossultan-pyrrha shows qualitatively a closer relationship 

 to H. ossultan than to II. pyrrha, but the reaction is very 

 close qualitatively in both parents and in the hybrid. 



The reaction with potassium sulphide begins in very 

 rare grains in 1 minute. Complete gelatinization occurs 

 in less than 0.5 per cent of the entire number of "grains 

 and of total starch in 5 minutes; still less than 0.5 per 

 cent of the grains and of total starch in 15 minutes; in 

 about 1 per cent of the grains and 3 per cent of the total 



starch in 30 minutes ; little if any further advance in 45 

 and 60 minutes, respectively. (Chart D 52.) 



The reaction with sodium hydroxide begins in a few 

 grains in half a minute. Complete gelatinization occurs 

 in about 2 per cent of the entire number of grains and 

 3 per cent of the total starch in 5 minutes ; in about 10 

 per cent of the grains and 27 per cent of the total starch 

 in 15 minutes; in about 23 per cent of the grains and 

 35 per cent of the total starch in 30 minutes ; in about 

 27 per cent of the grains and 43 per cent of the total 

 starch in 45 minutes ; in about 32 per cent of the grains 

 and 45 per cent of the total starch in 60 minutes. ( Chart 

 D53.) 



The reaction with sodium sulphide begins in a few 

 grains in 1 minute. Complete gelatiuization occurs in 

 about 0.5 per cent of the entire number of grains and 2 

 per cent of the total starch in 5 minutes ; in about 2 per 

 cent of the grains and 4 per cent of the total starch in 

 15 minutes ; in about 3 per cent of the grains and 6 per 

 cent of the total starch in 30 minutes ; in about 5 per cent 

 of the grains and 8 per cent of total starch in 45 minutes ; 

 little if any further advance in 60 minutes. (Chart D 54.) 



The reaction with sodium salicylate begins imme- 

 diately. Complete gelatinization occurs in about 18 per 

 cent of the grains and 22 per cent of the total starch 

 in 5 minutes ; in about 82 per cent of the grains and 85 

 per cent of the total starch in 15 minutes; in about 96 

 per cent of the grains and 98 per cent of the total starch 

 in 30 minutes; in more than 99 per cent of both the 

 grains and total starch in 45 minutes. (Chart D55.) 

 A small bubble appears at the hilum which in many of 

 the grains is not inclosed within a fissure; yet a cleft 

 appears at the hilum more frequently and expands to 

 greater size than in the parents; but more closely re- 

 sembles H. pyrrha. The definition of the lamella? and 

 the refractivity of the border is a little greater in both 

 cases than in //. ossultan, but about the same as in 

 H. pyrrha. The method of gelatinization is varied as 

 noted for the parents. The reaction proceeds from both 

 ends in many grains, a little less frequently than in 

 H. ossultan, but more frequently than in H. pyrrha. 

 In many grains the following is observed : The bubble at 

 the hilum is very resistant and expands to considerable 

 size; when gelatinization has almost reached the area 

 around the hilum, a tortuous fissure is formed which 

 forces its way through this region, the lamella? located 

 here often being disorganized by the appearance of re- 

 fractive granules. This method is similar to one observed 

 in the parents but the steps are more prominent, the 

 fissure more tortuous, and the appearance of refractive 

 granules not observed in the parents. 1 The fissures 

 which appear previous to gelatinization occur a little 

 more often than in H. ossultan, about the same as in 

 H. pyrrha. The gelatinized grains are swollen and dis- 

 torted so that they do not resemble the untreated grain, 

 as in both parents. 



In the reaction with sodium salicylate H. ossultan- 

 pyrrha shows qualitatively a closer relationship to the 

 parents, but is a little closer to H. pyrrha than to H. 

 ossultan. A character inherited from one parent is often 

 developed further in the hybrid. 



The reaction with calcium nitrate begins in rare 

 grains in 1 minute. Complete gelatinization occurs in 



