HIPPEASTRUM. 



423 



the color deepens quickly to moderately deep to very 

 deep, becoming somewhat hluer in tint. With 0.125 per 

 cent Lugol's solution the grains immediately color a light 

 blue-violet, lighter and less bluish than in H. dceones; 

 the color deepens quickly from moderate to moderately 

 deep, becoming distinctly bluer, a little lighter in color 

 and a little less reddish than in H. dceones. After heating 

 in water until the grains are gelatinized and, then adding 

 a 2 per cent Lugol's solution the grains color a light 

 to very deep blue, the majority with a reddish tint; the 

 color is lighter and more grains have a reddish tint than 

 in //. dceones. The solution becomes a very deep indigo- 

 blue, about the same as in H . dceones. If the preparation 

 is boiled for 2 minutes and then treated with an excess 

 of 2 per cent Lugol's solution the grain-residues color 

 a moderately light to moderately deep blue, the mean 

 is moderate, many having a slight reddish tint that is 

 lighter than in //. dceones. The capsules color a moder- 

 ately light to moderately deep heliotrope, lighter and less 

 reddish in tint than in H. dceones. The solution colors a 

 very deep indigo-blue, about the same as in H. dceones. 



ANILINE REACTIONS.. 



With gentian violet the grains stain very lightly at 

 once, a little lighter than in //. dceones, and in half an 

 hour they become moderate to moderately deep (value 

 55), a little lighter than in H. dceones. 



With safranin the grains color very lightly at once 

 and in half an hour they are moderate to moderately deep 

 in color (value 55), the same as in H. dceones. 



TEMPEBATUKE REACTIONS. 



The majority of the grains are gelatinized at 72 

 to 73 C., and of all but very rare grains at 73 to 

 75 C., mean 74 C., practically the same as H. dceones. 



EFFECTS OF VARIOUS REAGENTS. 



The reaction with chloral hydrate begins in 1 minute. 

 Complete gelatinization occurs in about 3 per cent of 

 the entire number of grains and 5 per cent of the total 

 starch in 5 minutes; in about 18 per cent of the grains 

 and 21 per cent of the total starch in 15 minutes; in 

 about 29 per cent of the grains and 32 per cent of the 

 total starch in 30 minutes; in about 32 per cent of the 

 grains and 36 per cent of the total starch in 45 minutes; 

 in about 36 per cent of the grains and 39 per cent of the 

 total starch in 60 minutes. ( Chart D 64.) One or more 

 bubbles, more frequently the former, appear at the hilum, 

 and an enlarged and refractive fissure a little less fre- 

 quently is seen at the hilum than in H. dceones. The 

 lamellae do not become more distinct as often as in H. 

 dceones. The entire grain becomes very refractive and 

 a border of slightly greater refractivity forms around 

 the margin, but it does not broaden so quickly as in 

 H. dceones. The methods of gelatinization are very simi- 

 lar to and quite as varied as in H. dceones. The pitted 

 appearance of the grain which may precede gelatiniza- 

 tion appears much less frequently and when present is 

 more generally localized at the distal margin. The 

 fissures which form during the process of gelatinization 

 are usually not quite so deep nor so branched. The 

 starch at the distal margin of the refractive border is 

 less frequently disorganized into refractive fragments 

 or granules previous to gelatinization. The gelatinized 

 grains are swollen and slightly to considerably distorted, 



a little more distorted than in H. dceones, and, as in this 

 species, they do not usually resemble the form of the 

 untreated grain. 



The reaction with chromic acid begins in a few 

 grains in 1 minute. Complete gelatinization occurs in 

 less than 0.5 per cent of the entire number of grains and 

 2 per cent of the total starch in 5 minutes; in about 

 10 per cent of the grains and 50 per cent of the total 

 starch in 15 minutes; in about 20 per cent of the grains 

 and 76 per cent of the total starch in 20 minutes; in 

 about 60 per cent of the grains and 85 per cent of the 

 total starch in 25 minutes; in about 86 per cent of the 

 grains and 95 per cent of the total starch in 30 minutes ; 

 in more than 99 per cent of both the grains and total 

 starch in 35 minutes ; a trace of starch at the margin in 

 a few grains is completely gelatinized in 45 minutes. 

 (Chart D 65.) 



The reaction with pyrogallic acid begins in a few 

 grains in 1 minute. Complete gelatinization occurs in 

 about 9 per cent of the entire number of grains and 11 

 per cent of the total starch in 5 minutes; in about 50 

 per cent of the entire number of grains and 68 per cent 

 of the total starch in 15 minutes; in about 80 per cent 

 of the entire number of grains and 93 per cent of the 

 total starch in 30 minutes ; in about 82 per cent of the 

 grains and 95 per cent of the total starch in 45 minutes ; 

 in about 88 per cent of the grains and 97 per cent of the 

 total starch in 60 minutes. (Chart D 66.) 



The reaction with nitric acid begins in a few grains 

 in half a minute. Complete gelatinization occurs in 

 about 5 per cent of the entire number of grains and 6 

 per cent of the total starch in 5 minutes ; in about 9 per 

 cent of the grains and 12 per cent of the total starch 

 in 15 minutes; in about 14 per cent of the grains and 

 45 per cent of the total starch in 30 minutes; in about 

 30 per cent of the grains and 60 per cent of the total 

 starch in 45 minutes ; in about 43 per cent of the grains 

 and 65 per cent of the total starch in 60 minutes. (Chart 

 D67.) 



A small bubble appears at the hilum and expands 

 even less, and a fissure is found a little less frequently at 

 the hilum than in H. dceones, but it becomes enlarged 

 and refractive as in that parent. The entire grain be- 

 comes more refractive and the lamellae do not usually 

 become more distinct with the exception of one which 

 forms a line of demarcation between the main body and 

 a border of slightly greater refractivity, and the defini- 

 tion of the lamellae and the demarcation of the main 

 body of the grain and its border is somewhat less sharp 

 than in H. dceones. The character and number of the 

 fissures formed during the process is the same and the 

 gelatinization of the grains is varied as in H. dceones, but 

 the gelatinization of the border previous to that of the 

 main body of the grain is more frequent, and the refrac- 

 tive granules formed during the reaction are more re- 

 sistant than in H. dceones. 



The gelatinized grains are swollen and slightly to 

 much distorted, as in H. dceones. Many grains are but 

 partially gelatinized, the same region usually being the 

 most resistant as in H. dceones. A number of grains 

 are but little affected by the reagent in 60 minutes, more 

 than in H. dceones. 



The reaction with sulphuric acid begins immediately. 

 Complete gelatinization occurs in about 55 per cent of 



