HIPPEASTRUM. 



427 



ref ractivity forms as in the parents ; it broadens a little 

 less rapidly than in H. dceones, and the same as in H. 

 zephyr. The methods of gelatinization are the same as 

 described under both parents. The pitted appearance 

 usually appears in the border, often at one point, and 

 less frequently over the entire surface of the grain than in 

 H. dceones, but more frequently than in H . zephyr. The 

 border of the grain is sometimes penetrated with moder- 

 ately deep irregular fissures which may break the border 

 into refractive fragments previous to gelatinization, as in 

 H. dceones, but more frequently than in H. zephyr. The 

 formation of fissures and of refractive granules during 

 the gelatinization of the grains is the same as in H. 

 dceones, a little more prominent than in H. zephyr. The 

 gelatinized grains are swollen and slightly to consider- 

 ably distorted, a little less distortion than in the parents ; 

 but a little closer to H . dceones than to H. zephyr in this 

 respect. 



In this reaction H. dceones-zephyr shows qualitatively 

 a very close relationship to both parents, but a little closer 

 to H. dceones in the more important steps. A character 

 observed in the parents is frequently developed further 

 in the hybrid. 



The reaction with chromic acid begins in 1 minute. 

 Complete gelatinization occurs in about 7 per cent of the 

 entire number of grains and 30 per cent of the total starch 

 in 5 minutes; in about .5 7 per cent of the grains and 80 

 per cent of the total starch in 15 minutes; in about 80 

 per cent of the grains and 90 per cent of the total starch 

 in 20 minutes; in more than 99 per cent of both the 

 grains and the total starch in 25 minutes ; only a portion 

 of the margin of a few grains remains ungelatinized. 

 Complete gelatinization occurs in 100 per cent of both 

 the grains and total starch in 30 minutes. (Chart D 65.) 



The reaction with pyrogallic acid begins in 1 minute. 

 Complete gelatinization occurs in about 15 per cent of 

 the entire number of grains and 17 per cent of the total 

 starch in 5 minutes; in about 67 per cent of the grains 

 and 80 per cent of the total starch in 15 minutes; in 

 about 75 per cent of the grains and 96 per cent of the 

 total starch in 30 minutes; in about 85 per cent of the 

 grains and 97 per cent of the total starch in 45 minutes ; 

 in about 93 per cent of the grains and 98 per cent of 

 the total starch in 60 minutes. (Chart D 66.) 



The reaction with nitric acid begins in a few grains 

 immediately. Complete gelatinization occurs in about 6 

 per cent of the entire number of grains and 7 per cent of 

 the total starch in 5 minutes ; in about 29 per cent of the 

 grains and 34 per cent of the total starch in 15 minutes; 

 in about 50 per cent of the grains and 73 per cent of the 

 total starch in 30 minutes ; in about 55 per cent of the 

 grains and 79 per cent of the total starch in 45 minutes ; 

 in about 60 per cent of the grains and 85 per cent of the 

 total starch in 60 minutes. (Chart D 67.) A small 

 bubble may appear at the hilum and have the same char- 

 acteristics as in the parents, but an enlarged fissure is 

 more frequently observed than in either parent, which 

 is a little closer to H. dceones. The entire grain becomes 

 very refractive and the lamella? frequently do not be- 

 come more distinct, but the definition of both the lamellae 

 in the main body of the grain and the one lamella forming 

 a line of demarcation between this region and a more 



refractive border becomes sharper than in either parent, 

 but nearer to H. dceones. The fissures formed during 

 the reaction are of similar character and number to those 

 noted in the parents. The gelatinization of the grains 

 is varied as in the parents, but the refractive border is 

 more frequently gelatinized previous to the main body 

 than in the parents, in which H. dceones-zephyr more 

 closely resembles H. dceones. The most resistant areas 

 are those noted in the parents, but the refractive granules 

 are less resistant than in the parent, in which H . dceones- 

 zephyr is closer to H. dceones. The gelatinized grains 

 are swollen and slightly to much distorted as in the 

 parents. Many of the grains are but partially gela- 

 tinized and some grains are but little affected at the 

 end of 60 minutes, though less than in the parents, in 

 which respect they more closely resemble H. dceones. In 

 this reaction H . dceones-zephyr shows qualitatively a very 

 close resemblance to the parents, but is a little closer to 

 H. dceones than to H. zephyr. A character appearing 

 in one parent is often further developed in the hybrid. 



The reaction with sulphuric acid begins immediately. 

 Complete gelatinization occurs in about 65 per cent of 

 the entire number of grains and 81 per cent of the total 

 starch in 5 minutes; in about 93 per cent of the grains 

 and 97 per cent of the total starch in 15 minutes; in 

 about 98 per cent of the grains and more than 99 per 

 cent of the total starch in 30 minutes. (Chart D 68.) 



The reaction with hydrochloric acid begins imme- 

 diately. Complete gelatinization occurs in about 6 per 

 cent of the entire number of grains and 8 per cent of the 

 total starch in 5 minutes; in about 38 per cent of the 

 grains and 70 per cent of the total starch in 15 minutes; 

 in about 54 per cent of the grains and 78 per cent of the 

 total starch in 30 minutes; in about 58 per cent of the 

 grains and 83 per cent of the total starch in 45 minutes ; 

 in about 78 per cent of the grains and 86 per cent of 

 the total starch in 60 minutes. (Chart D 69.) 



The reaction with potassium hydroxide begins imme- 

 diately. Complete gelatinization occurs in about 13 per 

 cent of the entire number of grains and 16 per cent of 

 the total starch in 5 minutes; in about 39 per cent of the 

 grains and 60 per cent of the total starch in 15 minutes ; 

 in about 53 per cent of the grains and 70 per cent of the 

 total starch in 30 minutes; in about 61 per cent of the 

 grains and 77 per cent of the total starch in 45 minutes; 

 in about 64 per cent of the grains and 83 per cent of the 

 total starch in 60 minutes. (Chart D 70.) 



The reaction with potassium iodide begins in a few 

 grains in half a minute. Complete gelatinization occurs 

 in but rare grains, less than 0.5 per cent of the entire 

 number of grains and 2 per cent of the total starch in 5 

 minutes; in about 3 per cent of the grains and 10 per 

 cent of the total starch in 15 minutes; in about 20 per 

 cent of the grains and 27 per cent of the total starch in 

 30 minutes; in about 23 per cent of the grains and 33 

 per cent of the total starch in 45 minutes; in about 30 

 per cent of the grains and 42 per cent of the total starch 

 in 60 minutes. (Chart D 71.) 



The hilum swells slightly and a bubble is occasionally 

 detected thereat, also the fissures when present at the 

 hilum become enlarged and more refractive; the pres- 

 ence of a bubble is more frequent and the fissures more 



