CRINUM. 



459 



of the total starch is gelatinized in 5 minutes. Up to 

 the end of 60 minutes little advancement of disorganiza- 

 tion is noted beyond a deepening of the longitudinal 

 fissures, especially in the distal region, and the beginning 

 of gelatinization in a few more grains. Complete gela- 

 tinization has occurred in about 0.5 per cent of the entire 

 number of grains and in 1 per cent of the total starch in 

 60 minutes. (Chart D 140.) 



The reaction with uranium nitrate begins in rare 

 grains in 1 minute. No grains are completely gelatinized 

 and but about 0.5 per cent of the total starch is dis- 

 organized in 5 minutes. Complete gelatinization 

 occurs in less than 0.5 per cent of the entire num- 

 ber of grains and 0.5 per cent of the total starch in 

 15 minutes. Slight progress of gelatinization is noted at 

 the end of 30 minutes, but less than 0.5 per cent of the 

 grains and 1 per cent of the total starch are gelatinized 

 at the end of 45 minutes, with no detectable progress at 

 the end of 60 minutes. (Chart D 141.) 



The reaction with strontium nitrate begins in rare 

 grains in 2 minutes. Gelatinization is observed in only 

 rare grains and in less than 0.5 per cent of the total starch 

 in 5 minutes. But little progress occurs in 15 minutes. 

 Complete gelatinization occurs in less than 0.5 per cent 

 of the entire number of grains and 1 per cent of the total 

 starch in 30 minutes; in about 1.5 per cent of the grains 

 and 2.5 per cent of the total starch in 45 minutes; and 

 in about 2.5 per cent of the grains and 3.5 per cent of the 

 total starch in 60 minutes. (Chart D 142.) 



The reaction with cobalt nitrate begins in very rare 

 grains in 1 minute. Complete gelatinization of any of 

 the grains was not observed, but the process begins in 

 rare grains, much less than 0.5 per cent of both the entire 

 grains and the total starch in 5 minutes. Very slight 

 progress occurs at periods of 15, 30, 45, and 60 minutes 

 respectively. At the end of 60 minutes complete gela- 

 tinization has occurred in but rare grains, and has begun 

 in but few others, the total amount of starch gelatinized 

 being less than 1 per cent. (Chart D 143.) The fis- 

 sures present in the untreated grain immediately become 

 much enlarged and much more refractive ; such fissures 

 not usually present in C. moorei. The enlargement and 

 refractivity are gradually lost, followed by a greater ex- 

 tension and branching of the longitudinal fissures. The 

 process of gelatinization begins either at the distal mar- 

 gin, or at one side near the distal margin, and is accom- 

 panied by considerable distention of the capsule, as noted 

 in C. moorei, but the reaction does not proceed proximal- 

 ward in nearly so many grains as in C. moorei, and the 

 most resistant parts are at the proximal end and sides 

 as noted for this species. 



The reaction with copper nitrate begins in very rare 

 grains in 1 minute. Complete gelatinization was not 

 observed in any of the grains, and the process has begun 

 in but rare grains, much less than 0.5 per cent in 5 

 minutes. Complete gelatinization occurs in but rare 

 grains and in about 0.5 per cent of the total starch in 5 

 minutes. There occurs little if any progress in 30, 45, 

 and 60 minutes respectively. At the end of 60 minutes 

 gelatinization has occurred in less than 0.5 per cent of 

 both the grains and the total starch. (Chart D 144.) 

 In very few of the larger grains the process begins at the 

 distal end of a deep fissure, and is accompanied by dis- 



tention and some ruffling of the capsule without proceed- 

 ing farther, the process beginning as noted for many 

 grains of C. moorei. In some of the smaller grains the 

 process extends through the mesial portion along the 

 course of well-defined fissures, as noted for some grains 

 of C. moorei, the distal margin undergoing gelatinization 

 previous to the proximal end and sides. 



The reaction with cupric chloride begins in very rare 

 grains in 1 minute. Complete gelatinization was not 

 observed in any grains and the process has begun in but 

 rare grains, much less than 0.5 per cent in 5 minutes. 

 Very slight progress occurs in .15, 30, 45, and 60 minutes 

 respectively. At the end of 60 minutes only about 0.5 

 per cent of the total starch is gelatinized. ( Chart D 145. ) 

 Gelatinization is complete in very few grains, in these the 

 process proceeds along the course of sharply defined, 

 longitudinal fissures, deeper and more sharply defined 

 than in C. moorei, the distal margin being comparatively 

 much more rapidly gelatinized than the proximal end. 

 In some grains this distal margin was affected much more 

 than the mesial portion and the process was accompanied 

 by extension and some ruffling of the capsule at the distal 

 margin as in C. moorei. 



The reaction with barium chloride begins in only rare 

 grains and by an enlargement and increased refractivity 

 of the clefts, and less than 0.5 per cent of the total starch 

 is gelatinized in 5 minutes. Very little change takes 

 place during 15 minutes, apart from a disappearance of 

 the refractivity, together with an extension of the clefts 

 at and proceeding from the hilum and the deepening of 

 fissures and extension toward the distal end. Even at the 

 end of 60 minutes only about 1 per cent of the total 

 starch is gelatinized. (Chart D 146.) 



The reaction with mercuric chloride begins in very 

 rare grains in 3 minutes. No complete gelatinization 

 in the entire number of grains was observed and the 

 process was begun in but rare grains, much less than 

 0.5 per cent, in 5 minutes. Very little progress occurs 

 in 15, 30, 45, and 60 minutes. At the end of 60 minutes 

 about 1 per cent of the total starch is gelatinized. ( Chart 

 D 147.) Treatment with this reagent is immediately 

 followed by an enlargement and greater refractivity of 

 the fissures that are present in the untreated grain. 

 Both this enlargement and refractivity are gradually 

 lost, but much more slowly than in C. moorei. This is 

 followed by an extension and greater branching of the 

 longitudinal fissures; but only rare grains are gela- 

 tinized, a very much smaller percentage than in C. 

 moorei. 



CRINUM HTBRIDUM j. c. HARVEY (HYBRID). 



(Plate 4, fig. 24; Charts D 127 to D 147.) 

 HISTOLOGIC PROPERTIES. 



The majority of the grains are simple in form and 

 isolated with the exception of a few which appear in 

 aggregates of from 2 to 5, generally 2 or 3, components. 

 Compound grains are fairly numerous and consist of 

 2 or 3 components. They are not so numerous as in 

 C. moorei, but about the same as in 0. zeylanicum. 

 Well-defined pressure facets were not observed. The 

 surface of the grain is often irregular owing to the same 

 causes as noted under the parents. They are not quite 

 so irregular as in C. moorei, but more so than in C. 

 zeylanicum. The occasional large blunt protuberances, 



