CRINUM. 



479 



proximal end and sides, but less frequently observed than 

 in both parents. A group of brilliant granules are 

 occasionally present in the area around the hilum and is 

 less frequently observed than in C. longifolium, but 

 more often seen than in C. moorei. They are somewhat 

 more distorted than in both parents, but like them bear 

 a general resemblance to the untreated grain. 



The qualitative reactions exhibit a somewhat closer 

 relationship in most of the grains to C. moorei than to 

 C. longifolium ; yet in some respects there is a decidedly 

 closer relationship to C. longifolium. 



The reaction with potassium sulphocyanate begins 

 immediately and a few grains are fully gelatinized in 

 30 seconds. Complete gelatinization occurs of all of the 

 grains except of the proximal end and sides of a few 

 grains in 1 minute; of about 93 per cent of the entire 

 number of grains and over 99 per cent of the total starch 

 in 3 minutes; with little further advance in 5 minutes. 

 (Chart D 177.) 



A bubble, which expands to considerable size and 

 is quite persistent in some grains, forms at the hilum. 

 It expands to greater size and is much less frequently in- 

 closed within an enlarged fissure than in C. longifolium j 

 it enlarges more, is more persistent, and a little more 

 frequently inclosed within an enlarged fissure than in 

 C. moorei. The lamellae frequently become more dis- 

 tinct than in C. longifolium, but less sharply defined 

 than in C. moorei. Fissures proceeding from the hilum 

 are formed in fewer grains than in both parents. The 

 lamellae are frequently disorganized through the mesial 

 region with the appearance of but slightly refractive 

 granules, much less refractive than in C. longifolium 

 and slightly less than in C. moorei; although in a few 

 grains the refractivity is closer to that in C. longifolium 

 than in C. moorei. The resistant starch is located at 

 the same area as in both parents. 



The gelatinized grains are swollen and much dis- 

 torted, more than in the parents, and they do not re- 

 semble the form of the untreated grain. 



The qualitative reactions with the exception of those 

 of a few grains, exhibit a closer relationship to C. moorei 

 than to C. longifolium. 



The reaction with potassium sulphide begins imme- 

 diately and very few grains are fully gelatinized in 1 

 minute. Complete gelatinization occurs of about 26 

 per cent of the grains and 60 per cent of the total starch 

 in 5 minutes ; of about 39 per cent of the grains and 74 

 per cent of the total starch in 15 minutes; of about 43 

 per cent of the grains and 85 per cent of the total starch 

 in 30 minutes; of about 45 per cent of the grains and 87 

 per cent of the total starch in 45 minutes ; and of about 

 50 per cent of the grains and 88 per cent of the total 

 starch in 60 minutes. (Chart D 178.) The Crinum 

 characteristic of a narrow very resistant border at the 

 proximal end is well shown in this starch by this reagent. 

 (See also C. longifolium and C. moorei.) 



At the hilum a bubble appears which enlarges more 

 than in either parent. The lamella in the mesial por- 

 tion become more sharply defined than in C. longifolium, 

 but about as in C. moorei. The refractive border at the 

 proximal end and sides is more quickly differentiated 

 into lamella? than in either parent. Much-branched fis- 

 sures appear similar to those of the parents, but their 



definition is more quickly lost. The lamellae of the mesial 

 margin are quickly disorganized, usually without the 

 appearance of granules, more frequently than in the 

 parents. A refractive border at the proximal end and 

 sides is the most resistant, as in the parents, and it is 

 gelatinized without breaking into granules more fre- 

 quently than in the parents. The grains are completely 

 gelatinized, they are much swollen, and usually much 

 distorted. They are considerably more distorted, and 

 the individual grains are to a larger extent gelatinized, 

 than in C. longifolium, but the mean distortion is about 

 the same, and a narrow striated band is less frequently 

 observed at the proximal end than in G. moorei. The 

 gelatinized grains do not resemble the forms of the 

 untreated grains. The qualitative reactions exhibit a 

 closer relationship to C. moorei than to C. longifolium. 

 The reaction with sodium hydroxide begins imme- 

 diately and many grains are fully gelatinized in 1 min- 

 ute. Complete gelatinization occurs of about 90 per cent 

 of the entire number of grains and 98 per cent of the total 

 starch in 3 minutes ; of about 95 per cent of the grains 

 and over 99 per cent of the total starch in 5 minutes; 

 and of all of the starch excepting traces of the proximal 

 end in rare grains in 10 minutes. (Chart D 179.) 



The reaction with sodium sulphide begins imme- 

 diately and many grains are fully gelatinized in 1 minute. 

 Complete gelatinization occurs of about 73 per cent of 

 the grains and 97 per cent of the total starch in 5 min- 

 utes ; of over 97 per cent of the grains and over 99 per 

 cent of the total starch in 15 minutes; and of all the 

 starch in 30 minutes. Only the proximal end of larger 

 grains and the extreme margin of a few smaller grains 

 exhibit resistant starch. (Chart D 180.) 



At the hilum a bubble appears which expands to a 

 large size, and is sometimes inclosed within an enlarged 

 fissure. It enlarges about as in C. longifolium, but is 

 less frequently inclosed within an enlarged cleft. It 

 is more persistent, enlarges to greater size, and is a little 

 more frequently inclosed within a cleft than in C. moorei. 

 The lamellae are more sharply defined and striated over 

 the entire grain than in the parents, the relationship 

 being less close to C. longifolium than C. moorei. Gela- 

 tinization occurs very rapidly without fissures being 

 observed, but when present they are of the same character, 

 though usually less sharply defined than in the parents. 

 This less closely resembles C. longifolium than C. moorei, 

 since the fissures of the latter are less frequent and deep 

 than the former. The mesial region is more frequently 

 gelatinized without the appearance of granules, but in 

 occasional grains a few quite refractive brilliant scattered 

 granules that are along the course of the fissures may 

 appear. The granules in most grains are much less 

 refractive than in C. longifolium, and there is greater 

 variation, with the mean a little lower than in C. moorei. 

 Either a refractive, distal, or marginal border is fre- 

 quently present, not quite so often as in C. longifolium, 

 and the lamellaa of which it is composed become more 

 clearly defined than in 0. longifolium. This border is 

 more frequently seen, especially around the entire mar- 

 gin, than in C. moorei. It is gelatinized without the 

 appearance of granules, as in the parents. The gelatin- 

 ized grains are much swollen and distorted, so that they 

 do not resemble the untreated grains, as in the parents. 



