NERINE. 



509 



in N. sarniensis var. corusca major, and they intersect 

 obliquely with the variation in the angle noted in those 

 grains. 



The degree of polarization varies from moderate to 

 very high (value 87), somewhat less than in 2V. sarniensis 

 var. corusca major as there are more grains having mod- 

 erately high and fewer having very high polarization than 

 in those grains. 



With sclenite the quadrants are more often well 

 defined and vary somewhat more in size and shape than 

 in 2V. sarniensis var. corusca major. The colors are 

 somewhat more pure and there are fewer grains having 

 a greenish tinge than in 2V. sarniensis var. corusca major. 



IODINE REACTIONS. 



With 0.25 per cent Lugol's solution the grains color 

 a moderately deep blue with a tinge of violet (value 

 65), more than in 2V. sarniensis var. corusca major, and 

 the color deepens very rapidly until it becomes deep to 

 very deep. With 0.125 per cent Lugol's solution the 

 grains color a light to moderate blue with a tinge of 

 violet, more than in 2V. sarniensis var. corusca major, 

 and the color deepens rapidly until it is deep. After 

 heating in water until the grains are gelatinized and then 

 adding a 2 per cent Lugol's solution the grains color 

 a moderate to very deep indigo-blue, more than in 2V. 

 sarniensis var. corusca major; the capsules can not be 

 distinguished; and the solution colors a deep indigo, 

 less than in 2V. sarniensis var. corusca major. If the 

 preparation is boiled for 2 minutes, and then treated with 

 an excess of 2 per cent Lugol's solution, the grain- 

 residues color a moderate to very deep indigo-blue with 

 a tinge of violet, deeper than in 2V. sarniensis var. corusca 

 major; the capsules of which fewer can be seen than 

 in 2V. sarniensis var. corusca major, a pure violet; and 

 the solution a very deep indigo as in 2V. sarniensis var. 

 corusca major. 



ANILINE REACTIONS. 



With gentian violet the grains stain lightly at once, 

 and in half an hour they are moderately stained (value 

 45), more than 2V. sarniensis var. corusca major. Some 

 grains are stained more than others and there is some 

 unevenness of coloring of the individual grains. 



With safranin the grains stain lightly at once, and in 

 half an hour they are moderately stained (value 45), 

 more than in 2V. sarniensis var. corusca major. Some 

 grains are stained more than others and there is some 

 uncvenuess of coloring of the individual grains. 



TEMPERATURE REACTIONS. 



The majority of the grains are gelatinized at 68.1 

 to 69 C., and all at 73.2 to 74.3 C., mean 73.8 C. 



EFFECTS OF VARIOUS REAGENTS. 



The reaction with chloral hydrate begins in a few 

 grains immediately. Complete gelatinization occurs in 

 about 13 per cent of the entire number of grains and 21 

 per cent of the total starch in 5 minutes; in about 84 

 per cent of the grains and 90 per cent of the total starch 

 in 15 minutes; in about 97 per cent of the grains and 

 98 per cent of the total starch in 30 minutes. (Chart 

 D232.) 



The hilum is as distinct as in 2V. sarniensis var. 

 corusca major, and the lamellae are at first indistinct but 

 later as distinct as in 2V. sarniensis var. corusca major. 



A broad refractive band, which is not so refractive as in 

 2V. sarniensis var. corusca major, is formed about the 

 margins of the grains. Gelatinization begins with about 

 equal frequency at the proximal and then at the distal 

 end, or first at the distal and then at the proximal 

 end, and in a moderate number of grains at either one 

 or the other end without immediate gelatinization of the 

 opposite end. Gelatinization progresses less smoothly 

 than in 2V. sarniensis var. corusca major and is accom- 

 panied by fissuring of the ungelatinized starch. The 

 most resistant part of the grain is just distal to the hilum,-. 

 differing from 2V. sarniensis var. corusca major in which 

 it was nearer the distal margin, and this may be split 

 into two pieces by a furrow from the hilum, or it may 

 be gelatinized in one piece. The gelatinized grains are 

 very large and considerably distorted and show less re- 

 semblance to the form of the untreated grain than in 

 2V. sarniensis var. corusca major. 



The reaction with chromic acid begins in rare grains 

 in 1 minute. Complete gelatinization occurs in much 

 less than 0.5 per cent of the entire number of grains 

 and total starch in 5 minutes; still in less than 0.5 per 

 cent of the grains and 3 per cent of the total starch in 

 15 minutes; in about 4 per cent of the grains and 36 

 per cent of the total starch in 30 minutes ; in about 38 per 

 cent of the grains and 85 per cent of the total starch in 

 45 minutes; in about 90 per cent of the grains and 

 more than 97 per cent of the total starch in 60 minutes. 

 (Chart D 232.) 



The reaction with pyrogallic acid begins in rare 

 grains immediately. Complete gelatinization occurs in 

 0.5 per cent of the entire number of grains and 1 per cent 

 of the total starch in 5 minutes; very slight progress in 

 15 minutes; in about 1 per cent of the grains and 2 

 per cent of the total starch in 30 minutes; very slight 

 if any further advance in 45 and 60 minutes, respec- 

 tively. ( Chart D 234.) 



The reaction with nitric acid begins in a few grains 

 immediately. Complete gelatinization occurs in about 

 2 per cent of the entire number of grains and 6 per cent 

 of the total starch in 5 minutes; in about 18 per cent 

 of the entire number of grains and 60 per cent of the 

 total starch in 15 minutes; in about 45 per cent of the 

 grains and 87 per cent of the total starch in 30 minutes; 

 in about 69 per cent of the grains and 90 per cent of 

 the total starch in 45 minutes ; in about 78 per cent of 

 the grains and 95 per cent of the total starch in 60 min- 

 utes. (Chart D 235.) 



The hilum and lamellae are very distinct, but less 

 than in 2V. sarniensis var. corusca major. Gelatinization, 

 as in 2V. sarniensis var. corusca major, begins at the 

 hilum, and an equal number of the grains are less resis- 

 tant at the proximal or at the distal end. The reaction is 

 accompanied by considerably more granulation and fis- 

 suring than in 2V. sarniensis var. corusca major, and in 

 the first-named starch the reaction is otherwise the same 

 as in 2V. sarniensis var. corusca major; but in the last- 

 named, 2 fissures extend from the hilum to the distal 

 end, branching out somewhat as they near the margin. 

 The starch included between the fissures becomes more 

 hyaline in appearance and is divided into granules by the 

 branching fissures, and as the hilum swells it is gela- 

 tinized from the hilum distally. The more resistant 



