512 



DATA OP PROPERTIES OF STARCHES OF PARENT- AND HYBRID-STOCKS. 



NERINE GLOKY OF SARNIA (HYBRID). 



(Plate 8. figs. 46 and 48; Charts D 232 to D 252.) 

 HISTOLOGIC PROPERTIES. 



In form the grains are usually simple and isolated, 

 and there are fewer compound grains and aggregates 

 than in either parent, in which respect this starch is 

 nearer that of N. sarniensis var. corusca major than 

 that of the other parent. When compound grains do 

 occur they are of the types noted under N. sarniensis var. 

 corusca major and a few such as are described under 

 N. curvifolia var. f other gilli major. They are more reg- 

 ular in form than either parent, and any irregularities 

 which occur are due to the following causes: (1) The 

 greater development of one part of the distal end or of 

 one side; (2) rounded protuberances from the sides or 

 the ends, usually from the proximal end; (3) secondary 

 sets of lamellae whose longitudinal axes are at angles to 

 the longitudinal axes of the primary sets; (4) notches 

 and depressions in the margin particularly at the distal 

 end; (5) deviation of the longitudinal axis with a 

 consequent bending of the grain. The conspicuous forms 

 are pure elliptical and elongated elliptical, and ovoid. 

 There are also triangular, clam-shell, pyriform, round 

 and nearly round, and lenticular forms. In form these 

 grains are slightly nearer N. sarniensis var. corusca 

 major. In form the parents and the hybrid are very 

 close. 



The hilum is as distinct as in N. sarniensis var. 

 corusca major, and is rarely fissured, if the fissures are 

 present they are of the same character as in N. sarniensis 

 var. corusca major. The hilum is sometimes centric, 

 but more often eccentric from 0.4 to 0.25, usually 0.32, 

 of the longitudinal axis, less than in either parent. In 

 character and eccentricity of the hilum those grains are 

 closer N. sarniensis var. corusca major than to 2V. fother- 

 gilli major. 



The lamellae are not so distinct as in either parent, 

 and there are more fine lamella? than in either parent. 

 In arrangement they resemble those of N. sarniensis var. 

 corusca major. The number counted on the larger grains 

 varies from 18 to 34, usually 28. In character, arrange- 

 ment and number of the lamella? these grains are nearer 

 N. sarniensis var. corusca major. 



In size the grains vary from the smaller which are 

 6 by 6/A and 10 by 8/x, to the larger elongated forms 

 which are 44 by 36/x, and 44 by 32/t, and the larger 

 rather rare) broad forms which are 46 by 42^,, in length 

 and breadth. The common sizes are 30 by 24/i and 30 

 by 20/t. The grains are closer N. curvifolia var. fother- 

 gilli major in size. 



POLARISCOPIC PROPERTIES. 



The figure is nearly centric to eccentric, usually as 

 eccentric and distinct and as clear-cut as in N. sar- 

 niensis var. corusca major. The lines are somewhat 

 less coarse than in N. sarniensis var. corusca major, the 

 same as in N. curvifolia var. fothergilli major; as in IV. 

 sarniensis var. corusca major they usually intersect 

 obliquely, with but little variation of the angle, and are 

 not often bent or bisected. 



The degree of polarization varies from moderate to 

 very high (value 90), the same as in N. sarniensis var. 



corusca major. It varies in different grains as in N. 

 sarniensis var. corusca major, a few having a moderate, 

 and the majority a high polarization. 



With selenite the quadrants as in N. sarniensis var. 

 corusca major are well denned, rarely of equal and 

 usually of unequal size, and vary somewhat in shape. 

 The colors are not quite as pure as in N. sarniensis var. 

 corusca major, but less pure than in N. curvifolia var. 

 fothergilli major, in this respect being closer to the 

 other parent. 



In degree of polarization, character of the figure, and 

 appearances with selenite N. glory of sarnia is closer 

 to N. sarniensis var. corusca major than to N. curvifolia 

 var. fothergilli major, although the two parents and the 

 hybrid all show a very close relationship. 



IODINE REACTIONS. 



With 0.25 per cent Lugol's solution the grains color 

 a moderate blue with a tinge of violet (value 55), less 

 than in N. sarniensis var. corusca major, and consider- 

 ably less than in N. curvifolia var. fothergilli major. 

 The color deepens rapidly until it is deep to very deep, 

 as in N. sarniensis var. corusca major. With 0.125 per 

 cent Lugol's solution the grains color a light to moderate 

 blue with a tinge of violet. The color is less than in 

 N. sarniensis var. corusca major and much less than in 

 N. curvifolia var. fothergilli major, and it deepens rap- 

 idly as in N. sarniensis var. corusca major. After heating 

 in water until the grains are gelatinized and then adding 

 a 2 per cent Lugol's solution, the grains color a moderate 

 to very deep indigo-blue the same as in N. sarniensis var. 

 corusca major; the capsules can not be distinguished ; and 

 the solution colors a moderately deep indigo-blue as in 

 2V. sarniensis var. corusca major. If the preparation 

 is boiled for 2 minutes, and then treated with an excess 

 of 2 per cent Lugol's solution, the grain-residues color a 

 light to very deep indigo-blue with a tinge of violet, as in 

 2V. sarniensis var. corusca major; the capsules pure violet 

 as in 2V. sarniensis var. corusca major; and the solution 

 a very deep indigo. Quantitatively and qualitatively the 

 iodine reactions of 2V. glory of sarnia are closer to 2V. 

 sarniensis var. corusca major than to 2V. curvifolia var. 

 fothergilli major. 



ANILINE REACTIONS. 



With gentian violet the grains stain lightly at once, 

 and in half an hour they are light to moderately stained 

 (value 35), less than either parent, but nearer to 2V. sar- 

 niensis var. corusca major. 



With safranin the grains stain lightly at once, and in 

 half an hour they are light to moderately stained (value 

 35), less than either parent, but nearer to 2V. sarniensis 

 var. corusca major. 



The aniline reactions of these grains show a closer 

 relationship to 2V. sarniensis var. corusca major than to 

 2V. curvifolia var. fothergilli major. 



TEMPERATURE REACTIONS. 



The majority of the grains are gelatinized at 70 to 

 72 C., and all at 75.8 to 77 C., mean 76.4 C. The 

 temperature of gelatinization of 2V. glory of sarnia is 

 somewhat closer to 2V. sarniensis var. corusca major than 

 to 2V. curvifolia var. fothergilli major. 



