692 



DATA OF PROPERTIES OP STARCHES OF PARENT- AND HYBRID-STOCKS. 



somewhat more numerous than in T. pottsii, but not 

 quite so frequently observed as in T. crocosmia aurea. 

 The conspicuous forms of the permanently isolated 

 grains are the same as in both parents, but more of the 

 ovoid are found than in T. pottsii, and the same number 

 as in T. crocosmia aurea. The small aggregates have the 

 same structure and number of components as noted for 

 both parents, but they more often consist of 1 large 

 and 1 small than in T. pottsii, but the same as in T. cro- 

 cosmia aurea. The grains, as in the parents, are not 

 flattened. This starch is somewhat closer T. crocosmia 

 aurea in form, although there are but slight differences 

 to be noted between the three. 



The kilum is a small, round or lenticular spot which 

 is more refractive than in T. pottsii, and the same as in 

 T. crocosmia aurea. Double hila are rarely observed, 

 as in both parents. A small, rounded cavity is more fre- 

 quently observed than in T. pottsii and as frequently as 

 in T. crocosmia aurea. The hilum is frequently fissured, 

 more often than in T. pottsii, and as frequently as in 

 T. crocosmia aurea. The structure of the fissures is 

 similar to those of both parents, being much more varied 

 and deeper than in T. pottsii, and as prominent as in 

 T. crocosmia aurea. Fissures extending distalward from 

 the hilum are more numerous, but of similar struc- 

 ture to those of both parents. The hilum is either 

 centric or eccentric from 0.45 to 0.3, commonly about 

 0.4, of the longitudinal axis. In eccentricity of the 

 hilum, T. crocosma 'flora is nearer T. pottsii, but in every 

 other characteristic it is nearer T. crocosmia aurea. 



The lamellce are not usually distinct, but are demon- 

 strable throughout the entire grain as often as in T. 

 pottsii, and somewhat more often than in T. crocosmia 

 aurea. The structure and the arrangement are the same 

 as noted for both parents, but the single coarse lamella 

 and the marginal band are more prominent than in 

 T. pottsii, but .not as markedly so as in T. crocosmia 

 aurea. The number on the larger permanently isolated 

 grains may be from 16 to 18, and on the larger separated 

 grains usually 12. In the character of the lamella? T. 

 crocosmceflora is somewhat nearer to T. pottsii, although 

 there are very few and minor differences to be noted 

 between the grains of the three starches. 



The size of the grains varies from the smaller which 

 are 3 by 2/*, to the larger permanently isolated grains 

 which are 32 by 27/*, and the larger dome-shaped separ- 

 ated components which are 28 by 22/t, in length and 

 breadth. The common size of the permanently isolated 

 grains is about 22 by 20/j. and of the separated about 20 by 

 20/i, in length and breadth. In size T. crocosmceflora is 

 nearer to T. pottsii than to T. crocosmia aurea. 



POLABISCOPIC PROPERTIES. 



The figure is usually centric to slightly eccentric, but 

 is quite eccentric in somewhat more grains than in T. 

 pottsii, but in fewer than in T. crocosmia aurea. The 

 lines are moderately fine and also intersect as in both 

 parents ; while they are straight with broadening towards 

 the margin in the majority of the grains, yet they are 

 moderately often bent and occasionally bisected as in 

 T. pottsii, but more often than in T. crocosmia aurea. 

 Both double and multiple figures are observed as in both 

 parents. 



The degree of polarization is high (value 67). The 

 grains vary from moderate to very high, with fewer 

 of the latter than in both parents, the mean being there- 

 fore slightly lower than in T. pottsii; and there is more 

 variation in the different grains, with the mean lower 

 than in T. crocosmia aurea. There is also considerable 

 variation in the same aspect of a grain, as in T. pottsii, 

 and more than in T. crocosmia aurea. 



With selenite the quadrants are usually well defined, 

 generally slightly unequal in size, and sometimes irregu- 

 lar, about as in T. pottsii, but neither quite so clean-cut 

 nor so regular as in T. crocosmia aurea. The blue is 

 generally pure, but the yellow is frequently not pure 

 throughout the entire quadrant, the same as in T. pottsii, 

 but not so pure as in T. crocosmia aurea. 



In the degree of polarization, the character of the 

 figure, and the reaction with selenite, T. crocosmceflora 

 is closer to T. pottsii than to T. crocosmia aurea. 



IODINE REACTIONS. 



With 0.25 per cent Lugol's solution most of the 

 grains immediately color a light reddish violet (value 

 25), which deepens somewhat rapidly with considerable 

 variation in the different grains, while a few remain color- 

 less, and with much more variation than in both parents. 

 The color is lighter and more reddish than that of the 

 few grains of T. pottsii which show any reaction with 

 this reagent; but the mean is considerably deeper, since 

 most of the grains remain colorless in T. pottsii. The 

 color is much lighter and is more reddish than in T. 

 crocosmia aurea. With 0.125 per cent Lugol's solution, 

 most of the grains remain colorless, while a few scat- 

 tered grains are a light blue-violet which quickly deepens 

 with a more bluish tint ; the majority are colorless as are 

 a few grains of T. pottsii, while the scattered grains are 

 of the same depth and tint as in T. crocosmia aurea. 

 After heating in water until all the grains are gelatinized 

 and then adding a 2 per cent Lugol's solution, the gelatin- 

 ized grains become moderately deep blue, some with red- 

 dish tint, deeper but near the tint of T. pottsii, and 

 deeper and more reddish in tint than in T. crocosmia 

 aurea. The solution becomes a deep indigo-blue, some- 

 what deeper than in T. pottsii, and as deep as in T. cro- 

 cosmia aurea. If the preparation is boiled for 2 minutes 

 and then treated with an excess of 2 per cent Lugol's 

 solution, the grain-residues become a moderate to deep 

 blue, many with reddish tint, not so deep as in either 

 parent, but as red as in T. pottsii and redder than in 

 T. crocosmia aurea. The capsules color a deep heliotrope 

 to wine-red, less of the latter coloring than in T. pottsii, 

 hence the color is not quite so deep nor so reddish as in 

 that species, but somewhat deeper and more reddish than 

 in T. crocosmia aurea. Qualitatively and quantitatively 

 the reaction with iodine shows a closer relationship to 

 T. pottsii than to T. crocosmia aurea. 



ANILINE REACTIONS. 



With gentian violet the grains immediately stain 

 lightly at once and in half an hour they are light to 

 moderately colored (value 40), the same as in T. pottsii, 

 but deeper than in T. crocosmia aurea. 



With safranin the grains immediately color lightly 

 and in half an hour they are light to moderate in color 

 (value 45), with a larger proportion of the latter than 



