776 



DATA OF PROPERTIES OF STARCHES OF PARENT- AND HYBRID-STOCKS. 



C. rosea, though much less frequent than in C. vestita 

 var. rubro-oculata. In the main body of the majority 

 of grains no lamellae are demonstrable, but this region is 

 heterogeneously refractive and is often bounded either by 

 an entire or a proximal and lateral more refractive bor- 

 der, in which 1 to about 3 marginal lamellae may be 

 clearly defined; such grains are less frequent, and the 

 main body less often inclosed within a refractive border 

 than in C. rosea, such grains were not observed in C. 

 vestita var. rubro-oculata. 



The lamellae are usually not demonstrable as in most 

 of the grains of C. rosea and the grains in which they 

 are demonstrable are closer to similar grains in C. rosea. 



The size of the grain varies from the smaller which 

 are 8 by 6/t, to the larger which are 36 by 24;u, com- 

 monly 21 by 16 n, in length and breadth. In size and 

 proportion the grains are closer to C. vestita var. rubro- 

 oculata. 



POLABISCOPIC PBOPERTIES. 



The figure varies from centric to quite eccentric, in 

 most grains it is not distinct and clean-cut, although 

 somewhat more frequently than in C. rosea, but much 

 less than in C. vestita var. rubro-oculata. A narrow 

 band at the margin of the majority of grains is arranged 

 like a box, with somewhat less frequency than in C. rosea, 

 but with very much more than in C. vestita var. rubro- 

 oculata. When the figure is clean-cut the lines vary 

 from moderately fine to coarse, more of the moderately 

 fine than in C. rosea, but much less than in C. vestita var. 

 rubro-oculata. The lines when distinct are arranged as 

 in both parents. 



The degree of polarization varies from low to very 

 high (value 60), and there is the same variation in the 

 different grains as in C. rosea, but more than in C. vestita 

 var. rubro-oculata. There is somewhat less variation in 

 the same aspect of a given grain, and hence the mean is 

 higher than in C. rosea, but there is usually much more 

 variation with the mean much lower than in C. vestita 

 var. rubro-oculata. 



With selenite the quadrants are usually not distinct, 

 excepting at the margin, though somewhat more fre- 

 quently in the typical grains than in C. rosea, but much 

 less frequently than in C. vestita var. rubro-oculata. 

 The colors are generally impure, about as in C. rosea, the 

 impurity usually indicated by an orange and purplish 

 color as in C. rosea. The scattered large grains with 

 sharply defined quadrants are more rare than in C. rosea; 

 when present the colors are usually tinged with green, as 

 in similar grains of C. rosea; the colors are much more 

 frequently impure than in C. vestita var. rubro-oculata. 



In degree of polarization, character of the figure, and 

 appearance with selenite the grains of G. veitchii are 

 closer to C. rosea. 



IODINE REACTIONS. 



With 0.25 per cent Lugol's solution the grains color a 

 moderate violet (value 43), a little deeper, bluer, and 

 with less variation than in C. rosea, slightly lighter and 

 less bluish than in C. vestita var. rubro-oculata; the 

 color deepens, rare large grains being deeper and slightly 

 bluer than the others, a little deeper, bluer, and with less 

 variation among the grains than in C. rosea, almost as 

 deep with a little more variation than in C. vestita var. 

 rubro-oculata. With 0.125 per cent Lugol's solution, the 



grains immediately color a light violet, they quickly be- 

 come moderately deep and bluer in tint with the rare larger 

 grains deeper and a little bluer ; most of the grains are a 

 little bluer and deeper than in 0. rosea, slightly lighter 

 and less bluish than in G. vestita var. rubro-oculata. After 

 heating in water until the grains are gelatinized and then 

 adding a 2 per cent Lugol's solution the gelatinized typi- 

 cal grains become a light to moderate blue with reddish 

 tint, lighter and more reddish than in both parents; 

 the rare large grains are a deep purplish-blue about as in 

 C. rosea, these grains are not observed in the prepara- 

 tion of G. vestita var. rubro-oculata; the solution becomes 

 a deep bluish-green, a little deeper and bluer than in 

 G. rosea, more greenish than in C. vestita var. rubro- 

 oculata. If the preparation is boiled for 2 minutes and 

 then treated with an excess of 2 per cent Lugol's solution 

 most of the grain-residues become a very light blue with 

 reddish tint, rare large grains moderately deep blue with 

 reddish tint, slightly lighter than in both parents; the 

 reddish tint nearer C. rosea than G. vestita var. rubro- 

 oculata; the capsules are moderate old-rose to brick-red, 

 lighter but very much the tint of C. rosea, lighter and 

 more yellowish in tint than in C. vestita var. rubro- 

 oculata. The solution becomes a very greenish-blue, less 

 greenish in tint than in C. rosea, but more than in C. 

 vestita var. rubro-oculata. Qualitatively and quantita- 

 tively the reaction with iodine is slightly closer to C. 

 rosea than to G. vestita var. rubro-oculata,. 



ANILINE REACTIONS. 



With gentian violet the grains color lightly at once, 

 some a little deeper than others, less variation than in C. 

 rosea, about as in C. vestita var. rubro-oculata; in 30 

 minutes they are moderate to moderately deep (value 

 57) with somewhat more of the latter, the mean is a 

 trifle deeper but nearly the same tint as in C. rosea; the 

 mean a little lighter but a little more reddish than in 

 C. vestita var. rubro-oculata. The same variation in 

 depth of the areas is present but less marked than in 

 C. rosea, but more prominent, with exception of the fis- 

 sures, than in C. vestita var. rubro-oculata. 



With safranin the grains color light to moderate at 

 once, the mean about the same as in the parents; in 30 

 minutes they become moderate to moderately deep, with 

 many more of the latter (value 65), the mean is a little 

 deeper than in C. rosea, and about the same depth as in 

 G. vestita var. rubro-oculata. The areas are about the 

 same as with gentian violet. 



The reaction with gentian violet is midway between 

 the two parents; that with safranin the same as that of 

 C. vestita var. rubro-oculata. 



TEMPERATURE REACTIONS. 



The majority of the grains are gelatinized at 71 

 to 72 C., and all at 73 to 74 C., mean 72.5 C. The 

 mean temperature of gelatinization is less than that of 

 either parent, and is closer to that of C. vestita var. rubro- 

 oculata. 



EFFECTS OF VARIOUS REAGENTS. 



The reaction with chloral hydrate begins immedi- 

 ately. Complete gelatinization occurs in about 60 per 

 cent of the grains and 80 per cent of the total starch in 

 5 minutes ; in about 94 per cent of the grains and 96 per 

 cent of the total starch in 15 minutes; in about 98 per 



