CONTRACTILITY OF VISCERA 5 



excised spleen to glass tube in the bath and to writing lever above, 

 lengthwise between them, as you did the gut. Warming the bath as before 

 observe the graphic record. Probably there is no beat. When the bath 

 reaches 35° C. add adrenalin as before, and observe the result. 



VI. Empty and rinse out the inner beaker. Refill it with fresh Ringer- Obs. 4. 

 Locke fluid and blow air through as before. From the piece of aorta taken from A<i^®^al extract 

 the cold Ringer-Locke fluid carefully cut a spiral band about 4 mm. wide and wall. 

 2-3 cm. long. Fix the exsected strip of aorta to glass tube and writing lever 

 as with spleen, and see that it is completely immersed in the bath. Put the 

 strip under stretch by hanging a 5 grm. weight to the writing lever at a distance 



Adr. 

 Aortcv 



!JllllJIJii/JWJMilUmiiJ(llllliiIiiiJMliittMli/iJliliai///lMli^ 



Text-fig. 4. Strip of exsected aorta (rabbit) in Kinger- 

 Locke fluid at 38° C; adrenal extract (E. Denniston). 

 T, time in 2" intervals. 



from the fulcrum similar to that of the attachment of the arterial strip but 

 on opposite side. Keep stretched for five minufes. Take off weight and let the 

 strip write a horizontal line for a couple of minutes. Apply flame to rewarm the 

 bath. Probably no beats will be seen. Add three drops of adrenal extract 

 solution as before when the temperature of the bath reaches 37° C. Observe 

 and record the result. 



