58 EXEKCISE VIII 



tissues on methylene-blue solution in Kinger-Locke fluid, a glass cannula with fine 



methylene-blue. nozzle for insertion into a vein and its glass stem fitting the rubber tube on 

 the burette. Set the methylene-blue solution to warm to 38° C. 



Expose the femoral vein at the groin, turning the fat-pad covering its upper 

 part upward and inward. Pass a thread ligature round the vein at its upper- 

 most part, a fine silk ligature loosely tied with the first bend of a reef-knot 

 round the vein about 6 mm. lower down, and a thread ligature again about 



4 mm. lower still. Pour the warm methylene-blue solution into the burette 

 and open pinch-cock to let out any air-bubbles. With the glass cannula ready 

 to hand, and with the top and bottom threads occluding the vein by a pair of 

 artery forceps attached to and dragging on them, open the vein just distal to 

 the middle ligature by a snip with fine scissors half through it. Insert the 

 cannula with nozzle pointing towards the heart. Tie tight the silk ligature 

 securing the neck of the cannula. Tie tight the distal ligature. Fill the 

 cannula with Ringer-Locke. Release the pinch-cock of the burette, preventing 

 outflow from burette by compressing the rubber tube near its free end between 

 finger and thumb. Slip the rubber tube over the free end of the venous 

 cannula, avoiding all air entering the connexion. 



Replace the pinch-cock on the rubber tube. Release fully the proximal 

 thread compressing the vein. Note the height of the methylene-blue solution 

 in the burette, and, releasing partially the pinch-cock, allow 5 c.c. to run into the 

 vein in about 1'. Feel the heart, and, if its beat is fairly strong, allow another 



5 c.c. to run from burette into vein ; and so on until 20 c.c. have been injected. 



Allow about 15' to elapse from end of injection. Open carefully the 

 abdomen along the median line ; note the colour of the intestine, spleen, one 

 kidney, the pancreas, the abdominal lymph-glands, and the muscles and 

 subcutaneous tissue of the abdominal wall. In doing this displace the viscera 

 as little as practicable. Then close the abdominal wound carefully and fully. 



Allow another 10'-15' to elapse. Reopen the wound, note the appear- 

 ances again, and extend the opening into the chest, to observe the colour of 

 the heart, lungs, and thymus gland. 



Then remove the artificial ventilation of the lungs. The heart will then 

 fail and the circulation cease. When that has happened perform a post-mortem 

 examination, making the following observations : 



Note that when first exposed certain organs are fully blue, namely, the 

 pancreas, thymus, lymphatic glands, and, to a less extent, the spleen ; that 

 certain others are not at all blue, namely, the lungs, the muscles, the liver, the 

 adrenal glands ; while others are slightly greenish-blue, e.g. kidneys and intestine. 



Take out rapidly one kidney, one adrenal, a piece of liver, the spleen, 

 a piece of the lung, and cut each open in turn. Note the blue mottling of the 



