68 EXEKCISE X 



V. Venous injection of Binger-Locke. While this observation is in progress, 

 fill the 50 c.c. burette in its vertical stand with Ringer fluid at 37° C, and 

 get rid of all air from it and from the rubber tube provided v^ith clamp attached 

 to its lower end. Bring the burette-stand into position near right side of neck, 

 so that its rubber tube can be connected with jugular cannula. Connect tube 

 with cannula, taking care to exclude air from junction and keeping clamp closed. 

 The level of fluid in burette should be at the top of the graduation of the 

 burette. 



VI. The 5' having elapsed and urinary flow, if any, been noted for that 

 time, open clamp on burette tube partially and allow 40 c.c. of the Ringer-Locke 

 to run slowly into vein. Observe urinary flow, if any ; probably there will 

 be none. 



Half fill burette again ; run in 25 c.c. more fluid slowly ; and observe 

 urinary flow, if any, for a further 5'. While this is in progress, fiUburette 

 with warm 10 per cent. NagSO^ solution, clamp being closed on burette. 



Obs. 43. ^11- Venous injection of 10 per cent, aqueous NagSO^ solution. The 5' being 



Renal secretion elapsed, open partially the clamp on burette-tube and run into the vein slowly 

 *i"om1^^^"^^^ ^^ ^'^- ^^^^ *^® burette. Observe urinary flow— drops begin to flow from 

 urethral tube in about 1' after injection. Count them minute by minute for say 

 10'; they may rise to 50-100 per min., but will have much diminished from that 

 in ten minutes' time. If no marked increase of flow has occurred in 5' give 

 another 10 c.c. of the NagSO^ solution, and look for diuresis again. 



Obs, 44. YIII. After an interval of 5' g ve by injection with needle-syringe 



Diuresis by into saphena vein a 5 c.c. dose of pituitary extract (' infundin ') and again 

 pituitary observe diuresis. Flow increases to perhaps 40 drops per min. 



Obs. 45. IX. After 10', during which the infundin-flow subsides, inject by jugular 



Diuresis by vein and cannula 10 c.c. of 1 per cent, solution of caffeine citrate. An increase 

 citrate^ ^^ urinary flow sets in about 2' later, and may amount to 20-40 drops 



per min. 



Obs. 46. X. Expose through an abdominal opening the right (or left) pelvic 



Detrusor action visceral nerve as it approaches the side of the neck and base of the bladder, 

 of n. peivicus. rpj^^ nerve is easily found by extending the abdominal incision freely, and 

 turning the bladder somewhat to one side. The nerve is seen low down on 

 the lateral aspect of the viscus. Ligate the nerve and cut it central to the 

 ligature. Faradize the distal piece lifted by the thread of the ligature. Note 

 the contraction of the bladder produced, and that it is unilateral, giving the 

 organ a curved asymmetrical appearance. 



