PANCKEAS AND SECRETIN 125 



the duct at a point between the two ligatures with fine-tipped scissors. 

 Steadying the duct by the ligature thread at duodenal end, pass nozzle of 

 cannula into the duct towards the gland and secure by tying its ligature. 

 Some semi- translucent colourless juice will enter the cannula at once. 



Cut away the piece of duodenum which has been separated from 

 pancreas. Your colleague prepares from this some more extract to add to the 

 filtrate (secretin) already made. Close abdominal wound carefully without 

 displacing duct cannula. 



, VII. Insert a venous cannula into the prepared {§ V, above) left external 

 jugular vein (see exerc. VI, § VII). 



VIII. Turn the preparation upon its right side. Reopen the abdominal 

 wound, and arrange pancreatic cannula so that it lies horizontal and is in line 

 with the duct itself. Observe the amount of juice in the cannula ; there has 

 probably been no addition to it since you left it. 



Inject into the jugular with glass syringe or from small burette, in either 

 case avoiding entry of air, 5 c.c. of the mucosa extract. Observe the juice 

 in the pancreatic cannula. About a minute after the injection of the extract 

 pancreatic secretion begins to flow. This will continue for 10' or so. Repeat 

 injection and observe again. 



IX. Inject into a saphena vein with the needle-syringe atropine sulphate 

 (see exerc. V, § VIII). Repeat the secretin injection into jugular. Observe 

 result. 



X. Open abdominal wound freely. Place a thread ligature round ^^®- ^^\ 

 common bile duct where it reaches surface of pancreas, taking care not to 



wound the portal vein ( Vp, text-fig. 42) ; leave the ligature loose. Tie another 

 ligature round it nearer to duodenum. Cut obliquely half through the duct 

 between the two ligatures. Insert a cannula directed towards liver and secure 

 with the first ligature. Bile flows into the cannula. Lift the cystic lobe of 

 liver and observe the gall-bladder. Compress the latter and collect the bile 

 from the cannula. Observe the colour and richly mucinous consistence of the 

 ' bladder-bile '. 



