PARENTERALLY INTRODUCED TRYPSIN 47 



method has been devised by P. v. Griitzner 35 on the principle 

 of his method of estimating pepsin. Fibrin is stained with 

 Spiritblau (diphenylrosanilin) and the color given to the 

 digestive fluid when the fibrin is digested in a 0.1 per cent, 

 soda solution is determined colorometrically. 



While the Schiitz-Borissow rule may be found applicable 

 within certain limits for the digestion of solid proteins, there 

 seems to exist for dissolved proteins a simple ratio between 

 the quantity of ferment and quantity of protein dissolved 

 by it. "Both groups," thinks Palladin, 36 "are correct, 

 those who believe that the Schiitz-Borissow rule obtains for 

 trypsin and those who think that Vollhard's rule of direct 

 ratio applies. It is not of consequence which method is used. 

 Probably the truth is that one ferment molecule does exactly 

 as much work as any other, that n molecules will do n 

 times as much as one in case no accidental inhibition occurs 

 and the ferment molecules can have full access to their prey 

 (if the term be permitted), that is to the protein, which is all 

 that Griitzner proved for pepsin originally." 



Toxicity of Parenterally Introduced Trypsin. Refer- 

 ence may here be made to a matter of general physiological 

 interest, the toxicity which parenterally introduced 

 trypsin or pancreatic tissue manifests upon the body. 

 In spite of the resistance of living tissues to digestive fer- 

 ments, mentioned in the preceding lecture, there unquestion- 

 ably is a decided production of necrosis when trypsin is in- 

 jected subcutaneously. When the pancreas of a dog under- 

 goes necrosis death quickly ensues, and that whether the 

 pancreas was that belonging to the animal or a foreign gland 

 transplanted under aseptic precaution. It is very interest- 

 ing, as discovered by Achalme, that animals may be im- 

 munized to a high degree against this toxic influence by 



35 A. Palladin (Physiol. Institut., Tubingen), Pfltiger's Arch., 134, 337, 

 1910; W. Waldschmidt, ibid., 143, 189, 1911. 

 3B P. Palladin, 1. c., p. 364. 



