ADRENIN IN BLOOD IN SUGAR PUNCTURE 307 



reduced. Section of the corresponding splanchnic nerve 

 protects the adrenal from these changes after glycosuric 

 puncture. ' ' 24 



Question of Increase of Adrenin After Sugar Puncture. 

 What of the third and most important of our postulates, on 

 which in fact the whole hypothesis stands or falls, the detec- 

 tion of an increased amount of suprarenin in the blood after 

 the sugar puncture? While Watermann and Smit 25 be- 

 lieved they had found an increase of suprarenin in the blood 

 after sugar puncture by means of the frog's eye method, not 

 only Kahn 26 but also E. Th. von Briicke 27 failed to recog- 

 nize such an increase. It should be said that the latter used 

 in determining the suprarenin in the blood serum the very 

 delicate Trendelenburg-Lawen test, which is based on the 

 idea that the vasoconstrictor power of a solution under ex- 

 amination may be tested by the variation in escape of fluid 

 when perfused through the hind legs of a frog. Kahn 28 has 

 objected to the force of this experiment because after sub- 

 cutaneous administration of suprarenin in small doses, but 

 sufficient to cause glycosuria, it is impossible, by available 

 methods, to recognize any accretion of this substance in the 

 blood. 



The author confesses that he is not entirely convinced of 

 the extent to which this objection is justified. For if the 

 suprarenin passes out from the adrenals into the circulation 

 in sugar puncture, it does so not by a subcutaneous route but 

 intravenously. As Kahn emphasized, adrenin-glycosuria 

 will appear in half-grown rabbits from doses of 0.1 milli- 

 gram upwards. A dose of this size given intravenously 



24 R. H. Kahn (Prague) , Pfliiger's Arch., 140, 254, 1911. 



25 N. Watermann and H. J. Smit, Pfliiger's Arch., 124, 198, 1908. 



26 R. H. Kahn (Prague), Pfliiger's Arch., 128, 519, 1909. 



27 E. Th. von Briicke (Leipzig), Mtinchener med. Wochenschr., 1911, No. 

 26; T. Negrin y Lopes (Physiol. Instil, Leipzig) , Pfliiger's Arch., 145, 311, 1912. 



28 R. H. Kahn (Prague), Pfliiger's Arch., 144, 251, 1912. 



