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clear liquid yielded in this case as distinct evidence of sugar as in the 

 other. Forty minutes after the death of the animal, another portion 

 of liver, which till then had remained undisturbed in the abdomen, 

 was treated like the others. This gave evidence of containing a much 

 greater quantity of sugar, thus confirming Bernard's statement, that 

 the transformation of glucogen goes on in the liver after its removal 

 from the body, or after the death of the animal. 



In order to be perfectly certain that the sugar found in the liver 

 at the instant of its removal from the body was really formed where 

 it was found, and not carried there by the portal blood from the 

 food, the following experiment was performed : 



Exp. 5. A dog was fed during ten days on boiled tripe. Twenty- 

 two hours after the last meal the animal was pithed. In less than 

 twenty seconds a portion of the liver was in the freezing mixture of ice 

 and salt. While I boiled directly another portion of liver, Professor 

 Sharpey put a ligature on the portal vein, and collected its blood. 

 He likewise collected some of the hepatic blood which flowed from 

 the cut liver. 



In the portal blood not a trace of sugar could be detected. The 

 hepatic blood, on the other hand, gave distinct evidence of its 

 presence. Both bloods were tested exactly alike. The clear liquids 

 obtained from the frozen liver and from the portion treated directly, 

 notwithstanding that they were filtered while hot, and also tested 

 while still hot, both gave distinct evidence of sugar. On the follow- 

 ing day a second portion of portal blood, which had been purposely 

 kept all night in order to ascertain if, on standing some time, sugar 

 would form in it, still yielded the same negative result. Even after 

 treating it with saliva, which would have transformed its glucogen 

 into sugar, had it contained any, no evidence of the presence of sugar 

 was obtained. On the other hand, when saliva was added to the 

 decoctions of the liver above spoken of, a great increase in the 

 amount of sugar was observed. The quantity of sugar so obtained 

 did not appear to be so great, however, as that yielded by a portion 

 of the liver which remained all night untouched in the abdomen of 

 the animal. 



Professor Garrod, F.R.S., who was present, not at the commence- 

 ment of the experiment, but on the following day, when the different 

 decoctions were tested, agreed with Professor Sharpey and myself, 



