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that this experiment showed the truth of Bernard's statement, that 

 the liver might contain both sugar and glucogeri when the portal 

 blood contained neither. 



The stomach and intestines of this animal were found void of 

 food ; the large intestine only contained fsecal matter. 



For the sake of still further assurance that the sugar found in the 

 liver was neither due to some accidental cause, nor immediately de- 

 rived from food, we determined to deprive an animal of food for 

 some days before examining the liver. The following experiment 

 was accordingly performed : 



Exp. 6. A very large and powerful dog, in admirable condition, 

 was subject to a rigid fast for seventy-two hours three full days. 

 Immediately after death, by section of the medulla oblongata, a por- 

 tion of the liver was sliced off and immersed in ice and salt. Blood 

 was then collected from the following sources : 



1st. From the portal vein. 



2ndly. From the liver (t. e. blood which flowed from the liver 

 when a portion of it was sliced off). 



3rdly. From the right side of the heart. 



4thly. From the aorta. 



5thly. From the inferior vena cava. 



Although these bloods were all treated in a similar manner, and 

 tested with the same quantities of copper and soda, yet none of them 

 gave unequivocal evidence of the presence of sugar, except that from 

 the liver. The blood from the right side of the heart gave doubtful 

 evidence. At first sight it may appear strange that the blood from 

 the right side of the heart should contain scarcely an appreciable 

 quantity of sugar, while that of the liver showed its presence very obvi- 

 ously ; but this no doubt arose from the hepatic blood being in great 

 part prevented from reaching the heart : 1st, on account of most of 

 it escaping into the abdomen, when the portion of liver was cut off ; 

 and 2ndly, on account of its flow being in great measure arrested by 

 the ligature of the portal vessels. 



All the bloods, except the hepatic, seemed to be free of glucogen 

 as well as sugar ; for none of them, with that exception, gave any 

 evidence of its presence after being treated with saliva in the usual 

 way. 



On examination of the frozen liver (after three hours), which, as 



