APPENDIX. 225 



January 17th, and observed for the first time on 

 March 3rd, forty-five days after being deposited; 

 the object being, as has already been stated in 

 1 The Field/ to test the suitability of the plan as 

 a means of effecting the transport of salmon ova 

 to Australia. The experiments were these : 



" First Box. The ova in this box were packed 

 between layers of moss, thoroughly saturated with 

 water, and placed in an ordinary meat safe. The 

 refrigerator was constantly supplied with ice in a 

 separate apartment, and kept at an uniform tem- 

 perature of 36. No water or ice or anything had 

 been added to this box, except once during the 

 second week, when a small piece of ice was placed 

 in the top of the box, which melted in a few hours. 

 On being opened, the ova appeared perfectly healthy, 

 and fewer were found dead than is generally the 

 case in the very best breeding-boxes. Mr. Frank 

 Buckland took a few out to try and hatch them in 

 order to prove their vitality, and he also placed 

 some of these ova in a bottle, with sponge inter- 

 vening, in the same way that he has received ova 

 from Huningue, to try the difference, if any, between 

 sponge and moss in the refrigerator. A few were 

 also given to Mr. Johnson to hatch. The box was 

 then carefully screwed down again, and replaced 

 under lock and key. 



" Second Box. The ova in this box were packed 

 in wet moss, and then placed in the large ice-box in 

 use at the Wenham Lake Ice Company's office, 

 140, Strand ; and upon the top of this box (which 

 was perforated with holes) ice was constantly placed. 

 As soon as one piece melted, it was replaced, and 

 the melted water, passing through the moss and 



