130 



THE GERM-CELLS 



serving as storehouses of material formed incidentally to the general 

 nuclear activity, but not of further direct use. 



Carnoy and Le Brim ('97, '99) reach, however, the conclusion that 

 in the germinal vesicle of Amphibia the chromosomes are derived 

 not from the chromatin-network, but solely from the nucleoli. The 

 apparent contradiction of this result with that of other observers is, 



* * 



;;v 



E 



Fig. 61. Germinal vesicles of growing ovarian eggs of the lamellibranch, Unio (A-D), and 

 the spider, Epeira (E-F). [OBST.] 



A. Youngest stage with single (principal) nucleolus. B, Older egg, showing accessory nucle- 

 olus attached to the principal. C. The two nucleoli separated. D. Much older stage, showing 

 the two nucleoli united. E. Germinal vesicle of Epeira, showing one accessory nucleolus at- 

 tached to the principal, and one free. F. Later stage ; several accessory nucleoli attached to the 

 principal. 



perhaps, only a verbal one; for the "nucleoli" are here evidently 

 chromatin-masses, and the disappearance of the chromatic network is 

 comparable with what occurs at a later period in the annelid egg 

 (Figs. 97, 128). 



2. The Cytoplasm 



The egg-cytoplasm varies greatly in appearance with the varia- 

 tions of the deutoplasm. In such eggs as those of the echinoderm 



