96 Mr. E. H. Hankin. [May 22, 



bottom of the vessel, and, before using it, the superabundant spirit is 

 poured off. The bottle is then shaken, and 5 to 10 c.c. of the turbid 

 liquid is poured out and filtered. The precipitate remaining on the 

 filter paper is washed with distilled water and then extracted with 

 about 15 c.c. of a -j^th saturated sodium sulphate solution. In other 

 cases it was at once extracted with 75 per cent, sodium chloride, or 

 with distilled water, the adherent salt enabling the precipitated 

 globulin to dissolve. 



Always the distilled water or other solvent employed had been 

 previously carefully sterilised. Only a very small part of the coagu- 

 lated mass of proteids was redissolved, the solution containing the 

 ferment-like cell globulin, while probably the other proteids present 

 were permanently coagulated by the action of the alcohol. To the 

 solution thus obtained a small quantity of anthrax culture is added. 

 The culture is generally in bouillon and not more than twenty-four 

 hours old. Before being added it has been violently shaken in order 

 to separate and break up the filaments. As soon as the anthrax 

 bacilli have been added to the globulin solution and shaken so as to 

 distribute them uniformly through the liquid, a sample of the latter 

 is taken, and with it a gelatine culture (which serves as control) is 

 made. This sample is not taken out with a platinum wire, but with 

 a capillary pipette. A kink is made in the pipette about 3 inches 

 from the end, and while removing samples of the liquid care is taken 

 that it should in each case be filled exactly to the kink. In this way, 

 as the same pipette is used throughout each experiment, it is easy to 

 abstract exactly the same quantity of liquid each time.* As soon as 

 the pipette has been emptied into the gelatine, the end that has been 

 used is placed in a test tube plugged with sterilised cotton-wool and 

 containing sterilised normal salt solution. This is immediately 

 boiled for a few minutes. Since the young culture of anthrax em- 

 ployed contains no spores, the pipette can be thus easily sterilised. 

 During the experiment the globulin solution is kept at a temperature 

 of 37. 



The following table shows some of my experiments in a tabular 

 form : 



* A separate pipette was used to inoculate the globulin solution with anthrax. 



