164 On the Influence of Bile on Pancreatic Digestion. [June 12, 



ferment action. A contained a deep yellow-coloured turbid fluid, 

 with a slight flocculent white precipitate ; B a light yellow-coloured 

 turbid fluid with copious white precipitate. The three mixtures were 

 then filtered through double, balanced filters, and the filter washed, 

 first with boiling distilled water, then with boiling methylated spirit, 

 and finally with absolute alcohol. The filters were then dried at 120 C. 

 and weighed. 



A, the fluid which contained bile salts, yielded a residue weighing 

 0'150 gram. 



B, the fluid which did not contain bile salts, yielded a residue 

 weighing O536 gram. 



C, which was not digested, gave a residue weighing 1'256 grams. 

 Experiment II. In this a larger proportion of bile salts was used, 



and digestion was conducted at a higher temperature. Equal quan- 

 tities of egg-albumen diluted with distilled water were introduced into 

 three beakers, A, B, and C, and coagulated by heat and a few drops of 

 acetic acid ; 1 per cent, of sodium carbonate was added to each, and to 

 A 3 per cent, of pig's bile salts ; to A and B 1 per cent, of pancreatic 

 extract rich in proteolytic ferment was added, and the mixture digested 

 at about 40 C. for three hours. The fluids were then boiled, and sub- 

 sequently filtered, the precipitates being washed and dried as in 

 Experiment I. The weights were as follows : 



A, the fluid which contained bile salts, yielded a residue weighing 

 0-098 gram. 



B, the fluid which did not contain bile salts, yielded a residue 

 weighing 0'065 gram. 



C, the fluid which was not digested, yielded a residue weighing 

 1'062 grams. 



Glycocholate of Soda. The effect of glycoholate of soda appeared 

 to be less marked than that of the bile salts as a whole. Thus, in 

 experiments conducted in the same manner as those above described, 

 a small portion of glycocholate of soda appeared to have the effect of 

 slightly increasing the amount of albumen dissolved, while a some- 

 what larger proportion either had a slightly contrary effect or none at 

 all. In one experiment equal quantities of albumen were taken, 

 and glycocholic acid,* 0'5 per cent., added to one vessel (C), and 

 I'O per cent, to another vessel (D), while none was added to a third 

 vessel (B), and the three fluids were then digested with pancreatin 

 for two hours. A fourth vessel was retained as control. The fluids 

 were filtered through balanced filter papers, and the filter washed 

 with distilled water, boiling methylated spirit, and absolute alcohol, 

 and dried at 110 C. 



The weights of the residues were as follows : 



* The acid was neutralised with 



