Experimental Investigation into the Pathology of Cancer. 401 



Death occurred about 36 hours after the experiment of ether 

 collapse of the lungs. 



Sheep (3). Beneath the subcutaneous fat of the back were placed 

 two grafts of a rapidly growing round-celled sarcoma removed from 

 the pectoral region for the third time. The grafts were cut in the 

 spray and then washed in warm sterile salt solution. The larger 

 graft was a thick slice about lj inch in diameter, and the smaller 

 was a cubical piece taken from the other half of the tumour. The 

 wound healed by first intention. On the 30th day the animal was 

 quite well, and on palpation no evidence of the grafts could be dis- 

 covered.* 



Besides the above transplantation experiments, we fed two white 

 rats, male and female, with portions of fourteen fresh scirrhus 

 tumours of the breast. In many instances the animals were fed on 

 two successive days with portions of the same scirrhus tumour. 

 These feeding experiments have extended over a period of seven 

 months, and the rats are now alive and well, 250 days from the date 

 of the first experiment. During this time several litters of young 

 rats have been born. -These feeding experiments we propose to 

 continue on the same animals. They were undertaken with the view 

 of seeing if infection of the stomach or intestine could be induced 

 in a way similar to that which is set up in some instances by the in- 

 jection of tuberculous material. 



In regard to the literature of the question, Scheuerlen, in the 

 autumn of 1887, read a paper before the Medical Society of Berlin, 

 claiming to have discovered a bacillus in cancer by means of arti- 

 ficial cultures. Francke corroborated this. But subsequent ob- 

 servers, amongst whom is Baumgarten (' Centralblatt f. Bakteriologie 

 n. Parasitenkunde,' vol. 3, No. 13), have shown that these observa- 

 tions were fallacious and due to contamination. Moreover, Rosen- 

 bach and Frankel (at Koch's request) kindly wrote and told us that 

 the swellings induced by Scheuerlen's operations were from their own 

 observations not cancerous. t 



Many experimenters have recorded observations with respect to 

 transplantation or grafting of cancer. Here again the results are at 



* July 8th. Animal killed 165 days after the experiment. Post mortem. No 

 sign of local or visceral infection. 



In the same way, between three and four months ago, we transplanted living 

 portions of scirrhus tumours into three white rats. At present they show no signs 

 of infection. It may be said, then, that at present there is no evidence that human 

 cancer can be transferred to brutes. July, 1890. 



t Professor Platon I. Kubasoff, of Moscow (' Proceedings of the third General 

 Meeting of Medical Men at St. Petersburg,' 1889, No. 2, p. 41) has asserted that a 

 bacillus can be cultivated on coagulated blood serum which will produce tumours in 

 rabbits and cats after inoculation. It will appear from what is stated in the teit 

 that this requires confirmation. 



