200 



Pre*ent*. 



[Feb. 12, 



Another method of staining consisted in the saturation of the tissue 

 with a copper salt and its redaction by sulphuretted hydrogen pre- 

 vious to coloration with anilinised alkalised acid fnchsin. 



By these staining methods the organisms have been demonstrated 

 in the blood, and also in the tissues. And some new, hitherto un- 

 recognised features are described, among which may be mentioned 

 what appears to be the germination of the spore in the blood, the 

 existence of a comma-shaped body and of mycelium in the spleen and 

 Peyer's glands, and the localisation of the plasmode, i.e., in relation to 

 the blood corpuscles. 



The isolation of the organism and its artificial cultivation have 

 been successfully carried out, and it is shown that this result entirely 

 depends for its success upon the fact that the nutrient media must be 

 previously treated with living blood, i.e., before rigor mortis has 

 set in. 



Alteration in the chemical composition of the nutrient medium, 

 consisting in the addition of glucose, together with iron or haemo- 

 globin or fresh blood, to the non-peptonised beef broth, elicited the 

 interesting fact that, under these circumstances, the organism can 

 pass to a more highly developed state, displaying the structure and 

 fructification of a highly organised fungus, but differing in certain 

 important features from any fungus hitherto described. 



Inoculation of guinea pigs, monkeys, and rabbits with the growths 

 in various nutrient media has produced a frequently fatal disease, 

 which, although not characterised in these animals by the symptoms 

 of classical intermittent fever, yet displayed in a number of instances 

 a definitely intermittent character. It was further, whatever its 

 clinical character, invariably accompanied by the appearance of the 

 characteristic organisms in the blood drawn after death from the right 

 ventricle. 



It is accordingly concluded that the malarial fungus is capable of 

 being cultivated outside the body and has been proved, to posse 

 pathogenic qualities. 



Presents, February 12, 1891. 

 Transactions. 



Brisbane : Royal Geographical Society of Australasia (Queenslant 

 Branch). Proceedings and Transactions. Vol. V. Part 2. 8\ 

 Brisbane 1890. The Society. 



Buenos Ayres : Museo de Productos Argentines'. Boletin Mensuj 

 Ano III. Num. 31. Resultados Botanicos de Esploracione 

 hechas en Misiones, Corrientes, <fcc., 1883-88. 8vo. [J 

 Aires'] 1890. The Muset 



Cambridge, Mass. : Harvard College. Museum of Comparative 



