1891.] Dr. F. Evans' Paper on the Fungus of Malaria. 539 



Preliminary experiments are first described bearing chiefly upon 

 the first-named source of error. 



Liebig's process for extracting kreatine from the juice of flesh was 

 modified by omitting the use of baryta-water, with the result that 

 abundance of kreatine was obtained, mixed with acid potassium 

 phosphate (KH 2 P0 4 ). In Liebig's process potassium chloride is 

 obtained after the kreatine has been separated. 



A preliminary experiment is then described in which the author 

 precipitated the albuminoid matters from the watery extract of fresh 

 butcher's beef by means of solution of mercuric chloride, the filtrate 

 depositing on standing a spherical precipitate, consisting of the 

 mercury salt of the sarcous kreatinin. from which a tabular kreatinin 

 was obtained isomorphous with the tabular kreatinin obtained by 

 the author from human urine in 1887. 



The special advantages of the method adopted by the author in 

 isolating the kreatinin of urine are next detailed, after which a series 

 of experiments are described in which muscle substance in different 

 stages of freshness was extracted with water, the extracts treated by 

 the mercuric chloride method, and. the products compared. 



Among these products is sarcous kreatinin, whose properties are 

 fully described and carefully compared with those of urinary 

 kreatinins previously investigated (vide 'Roy. Soc. Proc.,' vol. 43, 

 pp. 493-534). 



The final conclusion drawn is that sarcous kreatine is not present 

 in fresh muscle, but results from bacterial action, whereas sarcous 

 kreatinin is probably a true " educt." 



II. " Note on Dr. Fenton Evans' Paper on the Pathogenic 

 Fungus of Malaria." By W. T. TniSELTON DYER, M.A., 

 C.M.G., F.R.S. Received May 12, 1891. 



The abstract of this paper published in this volume of the ' Proceed- 

 ings ' contains (p. 200) the following statement : " Alteration in the 

 chemical composition of the nutrient medium . . . elicited the 

 interesting fact that, under these circumstances, the organism can 

 pass to a more highly developed state, displaying the structure and 

 fructification of a highly organised fungus, but differing in certain 

 important features from any fungus hitherto described." 



This statement will remain on record, and can hardly fail to cause 

 some perplexity to future students of the aetiology of malaria. 1 

 was present at the reading of the paper. The fungus exhibited was 

 undoubtedly " highly organised." It was in point of fact a typical 

 Mucor, and my friend Professor Marshall Ward, who was also 

 present, was disposed to regard it as identical with the form known 



VOL. XLIX. 2 



