On the Bases (Organic} in the Juice of Flesh. 293 



from these 30 Ibs. of beef were free from all danger of change by 

 bacterial action ; and the source from which the extract was made had 

 been exposed to bacterial action for only seven hours. This extract 

 will be spoken of as " portion A." 



The muscle fibre from which the above extract was taken was 

 left in a porcelain bath all night (the weather was very cold at the 

 time), and no more water was added to it. 



On Friday, January 4, this fibre was again put through the press, 

 and, although as much as possible had been squeezed out of it on the 

 previous day, and no more water had been added, an additional 

 3750 c.c. of a red liquid was obtained. 



The total volume of liquid obtained from these 30 Ibs. of flesh 

 amounted, therefore, to 6250 c.c., whilst the volume of water added 

 was 5000 c.c. Therefore, 1250 c.c. of actual juice must have been 

 expressed from 13'62 kilograms of flesh, i.e., the juice obtained was 

 about ^yth of the weight of the flesh taken. 



This second portion of juice was not mixed with portion A, -but 

 2250 c.c. of the mercuric chloride solution was added to it at 1 P.M. 

 on Friday afternoon, and it was filtered at once. The precipitate and 

 filtrate were preserved and labelled " Portion B." The utmost 

 exposure to bacterial action undergone by portion B was twenty-six 

 hours. 



So far there is evidence that one result of bacterial action is to 

 render the muscle substance more fluid. 



The following four portions of flesh from the same cow were 

 extracted with water, and the extracts mixed together in one large 

 vessel with mercuric chloride solution : 



(1.) 22 Ib. 8 oz. + 4 litres of water at 4 P.M. on Thursday 

 January 3, and left all night. 



Expressed 5220 c.c. of red liquid (of which 1220 c.c., or about ^th 

 of the flesh, must have been juice) at 3 P.M. on Friday, and added 

 2000 c.c. of HgCJ ^solution. 



(2.) 18 Ib. 12 oz. (= 8-576 kilograms) of flesh + 3 litres of water 

 at 1.30 P.M. on Friday, January 4. 



Expressed 3700 c.c. of red liquid (of which 700 c.c., or T L-th of 

 weight of the flesh, must have been juice) at 6 P.M. on Friday, 

 January 4, and added 2000 c.c. of HgCl 2 solution. 



(3.) 22 Ib. 8 oz. (= 10-215 kilograms of flesh) + 3 litres of water 

 at 4 P.M. Friday. 



3000 c.c. of red liquid squeezed out at 8 P.M. Friday, and 2000 c.c. 

 of HgCl 2 added. 



(4.) 22 Ib. 8 oz. of flesh + 3300 c.c. of water. 



Expressed 3100 c.c. of red liquid at 9 P.M. on Friday, January 4, 

 and mixed with 2000 c.c. of HgCl 2 solution. 



The juice from these last four portions was mixed with the mer- 



