On the Bases (Organic) in the Juice of Flesh. 290 



out adding anything to the solution. Pure lead hydrate was accord- 

 ingly added to all three filtrates. 



In portion A more time was required to remove all mercuric 

 chloride from the solution than in the case of portions B and C. 



Lead hydrate was first added to portion A in April, 1890 ; and, 

 although more lead hydrate was stirred in from time to time, the 

 solution was not free from mercury until March 23rd, 1891. It was 

 then filtered and evaporated. 



The filtrate (portion A was that which was extracted within seven 

 hours of the death of the animal) was evaporated first over steam, 

 then on a hot copper plate at 60 C. No brown colour was developed 

 in the solution during the concentration by heat, but it remained 

 colourless, even when reduced to a syrup. This concentrated liquor 

 was left standing over sulphuric acid. 



No kreatine crystals were formed in portion A, but a number of 

 octahedral crystals separated out. These crystals contain potassium, 

 chlorine, and much nitrogenous organic matter. Their solution does 

 not respond to Engel's test for kreatine with mercuric chloride and 

 potassium hydrate. 



The aqueous solution of these crystals is neutral to litmus. When 

 heated, the crystals lost 14' 7 per cent, of their weight, leaving a 

 black ash consisting of potassium chloride, entangling carbon. I 

 have a large quantity of these octahedral crystals, and I hope soon to 

 submit them to complete analysis and investigation. No P0 4 was 

 found in this part of portion A. 



In the case of portion B, the separation of the mercuric chloride 

 from the solution by lead hydrate was completed in three weeks. 



The filtrate was evaporated over steam. Only slight darkening of 

 colour took place during concentration. The yellow syrupy liquid 

 deposited crystals on statiding. The syrup was mixed with alcohol 

 (in which the crystals did not dissolve) and filtered. The crystalline 

 matter was washed with dilute alcohol, dried, and weighed ; its 

 weight was 2'24 grams. Having been weighed, the crystals were 

 redissolved and recrystallised, when they were found to be pure 

 KH 2 P0 4 . No kreatine crystals could be detected. The alcoholic 

 liquor was, of course, preserved for further examination. 



In the case of portion C, six weeks' digestion with lead hydrate 

 removed the mercuric chloride from the filtrate from the spherical 

 mercury salt of the sarcous kreatinin. The liquid was filtered from 

 the mixed lead and mercury precipitate, and concentrated by eva- 

 poration over steam and then at 60 C. 



Unlike portions A and B, portion C became extremely dark- 

 coloured during evaporation, and the product was a brown jelly, 

 entangling much crystalline matter. 



This residue was well stirred with dilute alcohol until only the 



