300 Mr. G. S. Johnson. 



crystals remained undissolved. The alcoholic liquor was then 

 filtered. The filtrate was preserved, and the crystals, having been 

 drained and washed with alcohol, were recrystallised from watery 

 solution. The recrystallised product was evidently kreatine, and its 

 weight was 16'63 grams. This kreatine was once more recrystallised 

 from water, and 0'2430 gram of the product (air-dried) was kept at 

 100 C. till its weight was constant. The previously transparent 

 crystals became opaque during this treatment, and lost 0'0290 gram 

 of H 2 0, or 11-93 per cent, of their weight. Kreatine, C 4 H 9 N 3 2 .H 2 0, 

 loses 12-08 per cent, at 100 C. 



Therefore, the portion of the extract of meat prepared from flesh 

 which had been most exposed to bacterial action became brown 

 during evaporation and deposited crystals of kreatine in abundance, 

 whilst the portions first extracted gave no kreatine, and did not 

 become brown during concentration. 



It will be well to summarise these results in tabular form. 



Portion A. 



Weight of flesh taken 30 Ib. (13'62 kilograms). 



Exposure to air before HgCl 2 added. ... 7 hours. 



Volume of cold saturated HgCl 2 added. . 3750 c.c. 



Weight of spherical Hg salt of kreatinin 44'16 grams. 

 Time required to remove HgCl 2 by 



Pb(HO) 2 11 months. 



Darkening of colour during evaporation 



by heat of filtrate after Pb(HO) 2 None. 



Kreatine obtained None. 



Crystalline product An octahedral com- 

 pound. 



Portion B. 



Weight of flesh (second extract from 



same portion of flesh as A) 30 Ib. (13'62 kilograms). 



Exposure to air before HgCl 2 added. ... 26 hours. 



Volume of HgCl 2 solution added 2250 c.c. 



Weight of spherical Hg salt of kreatinin 31'68 grams. 

 Time required to remove HgCl 2 by 



Po(EO), 3 weeks. 



Darkening of colour during concentration Very slight. 



Kreatine obtained None. 



Crystalline product KH 2 P0 4 . 



