1892.] On the Wandering (Migrating) Cell's of the Frog. 269 



The phenomena of leucoeytosis have also been examined, and we 

 find the following : 



1. Corresponding with the three different kinds of wandering cells 

 found in the blood and lymph, three kinds of leucoeytosis may be 

 distinguished, each characterised by the relatively greater increase in 

 number of one particular kind of cell. This may be illustrated by 

 citing the effect of the injection of finely-divided coagulated proteid, 

 which produces a great increase- in the number of the hyaline 

 (phagocytic) cells without a correspondingly large increase in the 

 numbers of the other wandering cell forms. Eosinophile leucoeytosis, 

 that is, increase in the numbers of the eosinophile cells, occurs with 

 wonderful rapidity after injection of anthrax bacilli or other micro- 

 organisms, and it is then followed by a leucoeytosis of the hyaline 

 cells. 



2. The leucoeytosis, or increase in the number of the cells, is 

 largely due to the proliferation of the cells themselves. Thus eosino- 

 phile leucoeytosis, followed by hyaline leu-cocytosis, occurs out of the 

 body in a hanging drop of lymph. Also we have witnessed the divi- 

 sion of the cells in a hanging drop. The phenomena classed under 

 the head of chemiotaxis are undoubtedly to be partly explained by the 

 very rapid power of proliferation by fission of the wandering cells. 



The behaviour of the cells towards micro-organisms differs according 

 to the nature of the latter. In this abstract we will confine ourselves 

 to the conflict with Bacillus anthracis. 



The Frog at ordinary temperatures is absolutely immune against 

 anthrax. When lymph is treated with anthrax bacilli the following 

 phenomena are seen, and may be grouped as successive stages : 



Stage I. The eosinophile cells are strongly attracted to the 

 anthrax. They apply themselves to the chains of bacilli. When 

 contact is absolutely or nearly effected their cell substance shows the 

 following phenomena : 



1. It is profoundly stimulated, and exhibits quick streaming move- 

 ments. Ordinarily the eosinophile cell is very sluggish. 



2. The eosinophile spherules are discharged : those nearest the 

 bacillus fading and dissolving first. 



3. If the eosinophile cells are present in sufficient numbers to 

 match the anthrax, in other words, if they are unharmed by the 

 bacilli, they bud off daughter cells, which are at first free from 

 granules. These creep a short way from the point of conflict, and in 

 a short time spherules appear at one end. Later, these daughter 

 cells seek the same or another focus of conflict. Several eosinophile 

 cells will, towards the close of Stage I, and when their numbers have 

 increased, be massed round one chain, and they ultimately fuse, 

 though the endosarc, with its granules, remains distinct. In this 

 way an eosinophile plasmodium is formed, though the fusion is con- 



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