272 On the Wandering (Migrating) Cells of the Frog. [Nov. 17, 



Action of Urari. 



It induces extensive leucocytosis. 



Stage I, After three Lours lymph drawn is found to contain 

 hyaline and amphophile cells, the latter in great abundance. 



By treating a hanging drop with urari and methylene-blue, we 

 were able to watch the granules of the eosinophile cells slowly 

 undergo a slight decrease in size and stain with the methylene-blue. 

 'The granules of the normal cell never stain with methylene-blue. 



Stage //, 12 hours. Repair in progress; numerous large cells 

 :present charged with ingesta. 



Stage III. The normal eosinophile cell re-appears. Frogs com- 

 pletely recover from urari in a day or two. 



Action of Heat. 



Frogs are rendered susceptible to anthrax by being warmed. We 

 therefore inoculated hanging drops and watched them on the warm 

 stage. 



We found that the first attack of the eosinophile cells was com- 

 menced before the temperature had risen, but never carried out, the 

 cells becoming completely paralysed, and showing no movement for 

 live hours. Therefore there was no phagocytosis, for this can only 

 jollow the eosinophile attack. 



Morphology and Comparative Physiology of these Wandering Cell 

 Elements. 



We are now able to point to three animal forms, the Frog and 

 Lamprey, types of a complex and highly developed group, and 

 Astacus, a complex member of a group containing animals of widely 

 divergent complexity. In all these different forms of wandering cells 

 occur. These we may class as 



Granular eosinophile. Found free in the body fluids. 



Non-granular hyaline. >} 



Rose-reacting cell, granular. Wandering cell which is found in the body fluids, 



but which also inhabits the spaces of connective 

 tissues, though it is not by any means identical 

 with the connective-tissue .cell. 



Of these diverse forms we see the archetype in the granular, pro- 

 tective, digestive, absorptive, and constructive (for it contributes to 

 form the fat tissue and scar tissue) blood cell of the primitive animal 

 Daphnia, and the granulation of this primitive cell is amphophile 

 and rose-staining, as is also the granulation of the ectoderm of 

 Daphnia. 



