1893.] Chemical Changes of Sugar injected into a Vein. 



185 



The liver is thus seen to contain more lactic acid than the muscles, 

 while, on the other hand, the blood sometimes contains more and 

 sometimes less than both the liver and muscles. 



From this it might be inferred that if the amount of a substance in 

 any given part of the body yields a clue to the seat of its formation, 

 it is probable that the liver is the principal seat of the lactic acid 

 formation, as pointed out by Wyssokowitzsch. 



(c.) Quantity of lactic acid excreted in the urine, the ureters having 

 been ligatured for six or more hours after the injection of sugar into 

 the jugular vein. 





From these results it appears that lactic acid is not only increased 

 in the blood after the injection of sugar, but that it is at the same 

 time excreted in the urine, whereas lactic acid is not known to 

 occur in healthy dog's urine. 



5. As acetone, acetoacetic acid, and ft-oxy butyric acid are often found 

 in the urine of diabetics, it was decided to examine it for these sub- 

 stances. 



(a.) Acetone and acetoacetic acid were found to be present in all the 

 five urines examined. 



(6.) p-Oxybutyric acid was searched for in the urine both with the 



