31 (> 



Dr. Noel Paton. 

 Per cent, of Glycogen in Liver. 



[Nov. 16, 



II. Fluoride of .sodium, in 1 per cent, solution, according to Arthus 

 and Huber, checks the action of protoplasm, but does not interfere 

 with the activity of zymins. It is shown that it markedly retards 

 hepatic amylolysis. It does not accelerate the structural changes in 

 the liver cells. 



III. Chloroform water, according to Salkowski, is a reagent which 

 stops the vital action of protoplasms, but does not interfere with the 

 action of zymins. 



He gives an experiment upon its influence on hepatic amylolysis, 

 from which he concludes that he has established the accuracy of 

 Bernard's view, that the conversion is due to a zymin. In a series of 

 experiments I have obtained results opposed to that of Salkowski. 

 Three portions of the liver were taken. One (A) was used for the 

 determination of the initial glycogen. The other two parts were 

 placed in separate vessels in normal saline at from 37 to 40 C. 

 Through one of these (B) a stream of chloroform vapour was con- 

 ducted, a stream of air being directed through the other (C). The 

 following results were obtained : 



Experiments were also made which show that the glycogen is 

 actually converted to glucose. 



Chloroform markedly increases hepatic amylolysis. 



To determine whether the early rapid or the latter slow conversion 

 is thus accelerated, the following experiment was performed. In it 

 the large letters indicate the presence of chloroform, the small letters 

 its absence : 



