98 Surgeon-Captain D. Brace. / [Mar. 11 



in Experiments 1 and 2, contained 159, 150, 171. and 168 polynuch 

 leucocytes in 20 fields of the fonr specimens examined ; the liu 

 J.'.'i and 209; the liver, 38 and 35 respectively. 



This experiment then shows a large increase in the number of 

 polynnclear leucocytes in the organs, as compared with what \v; 

 found in the organs of the control animals. 



But, as th injection of peptone causes not only a primary diminu- 

 tion but also a secondary augmentation in the number of lencoc 

 in the blood, it might be argued that this augmentation takes place 

 first in the organs, and that this might account for the increase foni 

 in Experiment No. 4. 



To meet this objection the following experiment was made : 



No. 5. Rabbit, 2 kilos. Blood from ear contained 9200 leucocyt 

 of which 47 per cent, or 4424, belonged to the polynuclear variety j 

 20 c.c. of a 10 per cent, peptone solution were now rapidly injc 

 into the left jugular vein, and the animal killed five seconds after 

 injection. 



The blood contained in the heart was at once examined, that in tl 

 left ventricle being found not to contain a single leucocyte of an] 

 kind whatever; that of the right only 166 per cubic millimetre, 

 told. It must be noted, however, that the leucocytes do not disapj 

 from all parts of the circulation with this marvellous rapidity, 

 Experiment No. 7 will show. 



The organs in this case were also found to contain a great exc 

 of polynnclear leucocytes : the spleen, 174, 133, and 149 ; the lui 

 230 and 221 ; the liver, 12 and 14. 



I next tried the effect of first causing a leucocytosis, and tl 

 driving the white blood corpuscles out of the blood by peptone, in 

 belief that some proportionate increase in the number of the polj 

 nuclear leucocytes in the organs might be found. 



N$. 0. Rabbit, weight 2 kilos. Blood from ear contained 7800 1< 

 cocytes, of which 4290 belonged to the polynuclear variety. 



Nineteen hours after the injection of 10 c.c. of a broth cultivatie 

 of anthrax, which had been sterilised by filtration through a Chaml 

 land filter, the number of leucocytes in the blood taken from the 

 had increased to 17,600, of which 11,264 were polynuclear. 20 

 of a 10 per cent, peptone solution were now injected into the 

 vein, and, five seconds afterwards, the animal killed. Blood fr 

 the left ventricle contained no leucocytes; that from the 

 ventricle only 1500 per cubic millimetre. 



The spleen sections contained 223 and 218, the lung 251 and 

 the liver 12 and 10. 



Experiment 7. Rabbit, 2 kilos. The blood taken from an 

 vein contained 14,300 white-blood corpuscles. 



20 c.c. of a 10 per cent, peptone solution were injected into 



