348 



Profs. Percy Frankland and Marshall Ward. 



Table J 



flask were made, and incubated also as carefully and long as possible, 

 to get the maximum numbers. 



The two plates from the exposed flask F 5 gave 160 and 320 after 

 four days' incubation. Total, 480 ; mean, 240 per c.c. ; whereas those 

 from the unexposed flask gave 3400 and 5916 respectively in the 

 same period. Total, 9316 ; mean, 4658 colonies per c.c. 



These numbers show very distinctly the effect of the sunshine, and 

 are borne out clearly by what follows. 



After the exposure on August 24th, and after the samples for plates 

 had been taken, both flasks were placed on ice over-night, and re- 

 mained on ice about fourteen hours. 



On tlie 25th, at 9.30 A.M., the exposure was repeated, the flasks 

 being first examined as to the effects of their sojourn in the ice-box. 

 The numbers were found to have remained remarkably constant, 

 being 210 for the exposed and 3136 for the covered flask. 



