360 Profs. Percy Frankland and Marshall Ward. 



This seemed to show that the exposure to what was, after all, only 

 diffused light, had very little effect in five and a half hours. 



Both flasks were taken in at 5.30, and put on ice for the night at 

 6 P.M., and remained on ice till 11 A.M. on the 21st, i.e., seventeen 

 hours in dark and on ice. They were then put out again from 

 11.15 A.M. to 4 P.M., the weather being much brighter, though plenty 

 of white cumulus clouds kept sweeping over the sun. 



Before putting out, two plates were made from each flask at 

 11.30 A.M. on the 21st. Those from V l (unexposed) gave 1,149,000 

 and 646,000 ; total, 1,795,000 ; mean, 897,500 ; numbers very 

 similar to those of the previous day, and indicating that no essen- 

 tial changes had occurred on the ice possibly a few had succumbed 

 to the rapid cooling. 



The two plates from the exposed flask F 2 gave 42,000 and 

 1,200,000 the last number being too high, as there were numerous 

 invading forms on the plate rendering it difficult to count. Taking 

 the figures as they stand we have, total, 1,242,000 ; mean, 621,000, 

 which is a reduction on last night's figures. 



After exposure on the 21st, two plates were again made from 

 each flask, with the following results. 



Of the two plates from the exposed flask F l5 one gave 122,500, 

 and the other 50,750 as the maximum numbers per c.c. Total, 

 173,250; mean, 86,625 per c.c. 



While the unexposed flask gave 1,920,000 and 640. The last low 

 number was obviously due to some blunder ; but, even if we take it 

 to reduce the average, we get total, 1,920,640 ; mean, 960,320 per c.c. 



After exposure, the flasks were again put on ice at 9 P.M., and 

 remained there till 12 noon next day, i.e., August 22 ; they had 

 been at 16 C. in the interval from 4.30 P.M. to 9 P.M. 



On the 22nd a plate was taken from each flask at 2.30 to 3 P.M., 

 and the F! gave no anthrax colonies at all, though nursed for nearly 

 a week. The other flask gave 990 colonies, which comes to 297,000 

 per c.c. 



On the 23rd, after another seven and a half hours' exposure, 

 plates were again made, one from each, and gave the following num- 

 bers the exposed flask 2 colonies, which = 720 per c.c.. and shows 

 that all spores were not yet killed, and the unexposed one 20 colo- 

 nies, which = 6800 per c.c. 



Unfortunately we were compelled to abandon these flasks now; 

 the mere labour of counting within the necessary periods the numer- 

 ous plates we were making made it imperative that this series should 

 be discontinued. I am now particularly sorry this was so, because 

 it would have been interesting to find if, and when, the light abso- 

 lutely cleared the water of spores. However, we could not foresee 

 what the tabular resume brings out so clearly. (Table 0.) 





