364 Profs. Percy Frankland and Marshall Ward. 



Table 0- 



energetic than has been commonly supposed, and the results also 

 suggest that some spores die off very quickly, even in the dark, when 

 put into sterile water a fact long known. 



On August 18, we started a similar experiment to the last, using 

 Thames water, freshly collected, instead of sterilised distilled water ; 

 but this had to be abandoned owing to the difficulties with the 

 rapidly-developing liquefying forms at the temperatures necessary 

 for growing the anthrax. There was nothing in the results to con- 

 tradict previous experience, but the details are of little value. 



XX. 



On October 6 I exposed a tube of broth, infected with a loopful 

 of colonies ft from gelatine stab-culture from 9 A.M. to 4 P.M. 

 (seven hours), over a mirror, to the sun ; the sky was clear, and sun 

 bright till about 1 P.M., and then duller. An exactly similar tube was 

 wrapped in foil and black paper, and placed by the side of the above. 



At 4 P.M. a plate was made from each tube, and incubated at 

 15 C. ; a stab-culture from each was also made and kept at 15 C., 

 and the original tubes were placed at 20 22 C. 



Taking the plates first. In forty hours the plate from unexposed 

 tube showed numerous colonies, from 0'5 mm. to 1 mm. diameter, 



