426 Profs. Percy Fraukland and Marshall Ward. 



became tuvbid, and then only after forty-eight hours, whilst the tube 

 in which only 0'5 c.c. of water was employed did not become turbid 

 at all. 



It must not, however, be supposed that the diagnosis of typhoid 

 bacilli in these waters was allowed to rest on such slender evidence as 

 the mere clouding of these phenol broth-cultures, but the latter were 

 submitted to gelatine plate cultivation to see if the characteristic 

 typhoid colonies made their appearance, and these colonies were 

 further confirmed by inoculation (a) on to potatoes for exhibition of 

 the characteristic growth, (&) into gelatine tubes to see if bubbles of 

 gas would make their appearance, (c) into broth for the indol test, 

 and generally also (cT) into milk to see whether coagulation of the 

 casein would take place. Thus in the case of the above phenol broth- 

 cultures commenced on 29.5.1893, the final confirmation of typhoid 

 was not obtained until 12.6.1893, or a fortnight later. 



The phenol broth test was again applied to the waters on 5,6.1893, 

 with the following results (p. 427). 



The plate cultivations made from the phenol broth tubes, referred 

 to in the table (p. 427), yielded the following results : 



Broth tube. 



(21.) Typhoid-infected Unsterilised Thames, Flask 1 I. (Typhoid 



Present.) 



The presence of typhoid was confirmed by the typical appear- 

 ance of colonies, growth on potatoes, negative indol test, and 

 negative gelatine bubble test. 



(23.) Typhoid-infected Unsterilised Thames, Flask 1 R. (Typhoid 



Present.) 



The presence of typhoid was confirmed by the typical colonies, 

 growth on potatoes, negative indol, and negative gelatine bubble 

 tests. 



(37.) and (45.) Coli-infected Unsterilised Thames, Flask 2 I. (B. 

 coli Present.} 



The presence of the B. coli was confirmed by typical colonies, 

 growth on potatoes, positive indol, and positive gelatine bubble 

 tests. 



(39.) and (47.) Coli-infected Unsterilised Thames, Flask 1 R. (B. 



coli Present.) 



The presence of the B. coli was confirmed by typical colonies, 

 growth on potatoes, positive indol, and positive gelatine bubble 

 tests. 



