428 



Profs. Percy Frarikland and Marshall Ward. 



Broth tube. 



(43.) Uninfected Unsterilised Thames, Flask 1 R. 



Colonies bearing some resemblance to typhoid yielded growths 

 on potatoes, which were also not unlike typhoid, but on inocula- 

 tion into gelatine tubes a green fluorescence was obtained, con- 

 clusively proving that it was another organism and not typhoid 

 or coli. 



From these examinations then it was apparent that on this day, 

 5.6.1893 



1. The unfiltered Thames water infected with typhoid on 11.5.1893, or 

 twenty-five days previously, still contained living typhoid bacilli, both in 

 that portion of the water which had been preserved at 19 C. as well as in 

 that kept at 6 C., the number and vital activity of the typhoid bacilli 

 being apparently greater in the latter than in the former. 



2. The unfiltered Thames water infected with the B. coli communis 

 on the same date also still contained these bacilli in a living state, both 

 in that portion of the water kept at 19 C. as ivell as in that maintained at 

 6G. 



3. The unfiltered uninfected Thames water ivhich had been maintained 

 under exactly similar conditions, contained no bacteria which after careful 

 examination could be mistaken either for the typhoid bacillus or the B. 

 coli communis. 



The unfiltered infected waters were again examined on 14.6.1893, 

 with the following results : 



Examination by Phenol Broth-culture on 14.6.1893. 



The plate cultivations made from the above phenol broth tubes 

 gave the following results : . 



